Table 1 Hotspot-activating DNA sequence motifs and their binding proteins
Motif nameMotif sequenceaBinding proteins
M265′-ATGACGT-3′Atf1-Pcr1
CCAAT5′-CCAATCA-3′Php2-Php3-Php5
Oligo-C5′-CCCCGCAC -3′Rst2
40955′-GGTCTRGACC-3′Unknown
41565′-TCGGCCGA-3′Unknown
  • a Experimentally defined core sequences that are sufficient to promote recombination locally; R = A or G. The base pair substitutions used to generate these DNA binding sites in the ade6 gene and the DNA sequences of all other ade6 alleles used in this study are provided in Table S2.