Table 1 Frequency of reversible silencing of URA3-integrated strains
Integration typeTransformant no.No. of reversibly silenced colonies/total no. of 5-FOA colonies analyzedPercentage reversible silencing of URA3 (%5-FOARURI+HIS+ARG+)
L510/107ND
20/73ND
30/95ND
L411/1160.862
20/103ND
31/1170.854
L311/861.162
22/962.083
32/982.04
L211/971.03
23/1172.564
30/100ND
L1111/11010
223/15814.556
35/1603.125
CEN7::URA3/CEN7J15172/7497.297
J15346/6178.688
J15475/7994.936
R1199/10198.019
258/58100
371/7891.025
R212/1181.694
21/1110.9
31/1001
R311/1110.9
21/1080.925
31/881.136
R413/973.09
21/961.04
32/1011.98
R511/1380.724
22/1571.273
30/100ND
far-CEN710/200ND
20/200ND
30/205ND
CEN5 int14/984.08
22/1141.75
30/89ND
  • ND, not detected.