Table 1 Ten novel Hprt targeted human MaxiPromoter strains generated
Chosen forGenePleParental BAC (RP11-)BAC size (bp)Retrofit-ted BAC (bEMS)Targeted ESC line (mEMS)Strain name (B6.Cg-)JAX Stock #
ThalamusSLITRK6252398A22197,3721685708aHprttm340(Ple252-icre/ERT2)Ems023685
Basal gangliaAGTR1270487M24231,1902375930bHprttm343(Ple270-icre/ERT2)Ems023688
Blood brain barrierCLDN527216C10197,6271915806aHprttm332 (Ple272-icre/ERT2)Ems023677
PvHCRH2741006F7205,6671925758aHprttm353 (Ple274-icre/ERT2)Ems023698
CortexHTR1B277990K4196,5042285794aHprttm335 (Ple277-icre/ERT2)Ems023680
StriatumKCNA427863H13191,7832406120bHprttm357 (Ple278-icre/ERT2)Ems023702
HippocampusNEUROD62811087H14209,9311935724aHprttm333 (Ple281-icre/ERT2)Ems023678
HippocampusNPY2R283937K11188,6182415899bHprttm354 (Ple283-icre/ERT2)Ems023699
Neural stem cellsSOX32861019M14208,4792445931bHprttm355 (Ple286-icre/ERT2)Ems023700
HypoxiaSPRY12871126D8209,1972486107aHprttm358 (Ple287-icre/ERT2)Ems023703
  • BAC, bacterial artificial chromosome; bEMS, BAC Elizabeth M. Simpson; ESC, embryonic stem cell; JAX, The Jackson Laboratory; mEMS, mammalian cell line Elizabeth M. Simpson; Ple, Pleiades promoter; PvH, paraventricular hypothalamic nucleus.

  • a mEMS4857 was the parental ESC line.

  • b mEMS4855 was the parental ESC line.