Table 2 17 novel Hprt targeted MiniPromoter strains generated

Chosen for	Gene/genomic region	Ple	MiniP size (bp)	Plasmid name (pEMS)	Targeted ESC line (mEMS)	Strain name (B6.Cg-)	JAX Stock #
Raphe nuclei	FEV	67	2202	2011	6017^{^a}	Hprt^{tm366a(Ple67icre/ERT2)Ems}	023711
Gabaergic neurons	GPR88	94	3049	2014	6029^{^a}	Hprt^{tm359a(Ple94-icre/ERT2)Ems}	023704
Purkinje, bipolar	PCP2	155	1652	2004	5985^{^a}	Hprt^{tm347a(Ple155-icre/ERT2)Ems}	023692
Raphe nuclei	SLC6A4	198	2826	2015	6044^{^a}	Hprt^{tm351a(Ple198-icre/ERT2)Ems}	023696
Thalamus	TNNT1	232	1209	2010	6006^{^a}	Hprt^{tm350a(Ple232-icre/ERT2)Ems}	023695
Cortex	C8ORF46	251	2453	2002	5937^{^a}	Hprt^{tm367a(Ple251-icre/ERT2)Ems}	023712
Substantia nigra	PITX3	253	2484	2003	5929^{^a}	Hprt^{tm360a(Ple253-icre/ERT2)Ems}	023705
Neurogenic, müller glia	NR2E1	264	3026	2006	6004^{^b}	Hprt^{tm346a(Ple264-icre/ERT2)Ems}	023691
Bipolar	PCP2	265	986	2005	5991^{^a}	Hprt^{tm348a(Ple265-icre/ERT2)Ems}	023693
Glia, astrocytes	S100B	266	2982	2007	5950^{^a}	Hprt^{tm365a(Ple266-icre/ERT2)Ems}	023710
Glia, oligodendroglia	UGT8	267	3014	2009	5970^{^a}	Hprt^{tm361a(Ple267-icre/ERT2)Ems}	023706
Glia, oligodendroglia	OLIG1	304	2596	2018	6089^{^a}	Hprt^{tm362a(Ple304-icre/ERT2)Ems}	023707
Forebrain	hs671^{^c}	N/AP	2129	2016	6068^{^a}	Hprt^{tm349a(hs671-icre/ERT2)Ems}	023694
Midbrain	hs1218^{^c}	N/AP	2338	2017	6070^{^a}	Hprt^{tm363a(hs1218-icre/ERT2)Ems}	023708
Minimal promoter control	Hspa1a^{^c}^,^{^d}	N/AP	878	2012	6051^{^a}	Hprt^{tm352a(Hspa1a-icre/ERT2)Ems}	023697
Ubiquitous control	CAGGS	N/AP	1723	2013	6096^{^a}	Hprt^{tm364a(CAGGS-icre/ERT2)Ems}	023709
Promoterless control	(none)	N/AP	0	2001	5895^{^a}	Hprt^{tm345a(icre/ERT2)Ems}	023690

ESC, embryonic stem cell; JAX, The Jackson Laboratory; mEMS, mammalian cell lines Elizabeth M. Simpson; MiniP, MiniPromoter; N/AP, not applicable; pEMS, plasmid Elizabeth M. Simpson; Ple, Pleiades promoter.
↵a mEMS4855 was the parental ESC line.
↵b mEMS4857 was the parental ESC line.
↵c Genomic regions from the VISA Enhancer Project (https://enhancer.lbl.gov/).
↵d Synonym Hsp68.