Table 2 17 novel Hprt targeted MiniPromoter strains generated
Chosen forGene/genomic regionPleMiniP size (bp)Plasmid name (pEMS)Targeted ESC line (mEMS)Strain name (B6.Cg-)JAX Stock #
Raphe nucleiFEV67220220116017aHprttm366a(Ple67icre/ERT2)Ems023711
Gabaergic neuronsGPR8894304920146029aHprttm359a(Ple94-icre/ERT2)Ems023704
Purkinje, bipolarPCP2155165220045985aHprttm347a(Ple155-icre/ERT2)Ems023692
Raphe nucleiSLC6A4198282620156044aHprttm351a(Ple198-icre/ERT2)Ems023696
ThalamusTNNT1232120920106006aHprttm350a(Ple232-icre/ERT2)Ems023695
CortexC8ORF46251245320025937aHprttm367a(Ple251-icre/ERT2)Ems023712
Substantia nigraPITX3253248420035929aHprttm360a(Ple253-icre/ERT2)Ems023705
Neurogenic, müller gliaNR2E1264302620066004bHprttm346a(Ple264-icre/ERT2)Ems023691
BipolarPCP226598620055991aHprttm348a(Ple265-icre/ERT2)Ems023693
Glia, astrocytesS100B266298220075950aHprttm365a(Ple266-icre/ERT2)Ems023710
Glia, oligodendrogliaUGT8267301420095970aHprttm361a(Ple267-icre/ERT2)Ems023706
Glia, oligodendrogliaOLIG1304259620186089aHprttm362a(Ple304-icre/ERT2)Ems023707
Forebrainhs671cN/AP212920166068aHprttm349a(hs671-icre/ERT2)Ems023694
Midbrainhs1218cN/AP233820176070aHprttm363a(hs1218-icre/ERT2)Ems023708
Minimal promoter controlHspa1ac,dN/AP87820126051aHprttm352a(Hspa1a-icre/ERT2)Ems023697
Ubiquitous controlCAGGSN/AP172320136096aHprttm364a(CAGGS-icre/ERT2)Ems023709
Promoterless control(none)N/AP020015895aHprttm345a(icre/ERT2)Ems023690
  • ESC, embryonic stem cell; JAX, The Jackson Laboratory; mEMS, mammalian cell lines Elizabeth M. Simpson; MiniP, MiniPromoter; N/AP, not applicable; pEMS, plasmid Elizabeth M. Simpson; Ple, Pleiades promoter.

  • a mEMS4855 was the parental ESC line.

  • b mEMS4857 was the parental ESC line.

  • c Genomic regions from the VISA Enhancer Project (https://enhancer.lbl.gov/).

  • d Synonym Hsp68.