Table 2 Quantification of nuclear abnormalities in embryos from HP1a-deficient mothers
Maternal genotypeMultiple nuclei Overcondensed nucleiAsynchronous nuclear divisionn
w11188 (0.0%)4 (0.0%)14 (0.1%)268
Su(var)2-5048 (0.0%)6 (0.0%)6 (0.0%)324
Su(var)2-504; HP1a RNAi#1286 (40.6%)386 (54.8%)484 (68.8%)704
Su(var)2-504; EGFP RNAi#14 (0.0%)2 (0.0%)12 (0.0%)400
Su(var)2-504; HP1a RNAi#2354 (64.8%)180 (65.9%)450 (82.4%)546
Su(var)2-504; EGFP RNAi#26 (0.0%)2 (0.0%)16 (0.0%)506
  • For each maternal genotype, the percentage of embryos with the indicated phenotypes was calculated from the total number (n) counted in two independent experiments. “Multiple nuclei” indicates fragmented nuclei. The total number counted (n) for HP1a and H3K9me3 was the same (counted from costained preparations) and are presented in a common column between the two. The percentage reported in the Results section is the average percentage from those of HP1a RNAi #1 and HP1a RNAi #2. RNAi, RNA interference; EGFP, enhanced GFP.