Table 1 3×FLAG tag insertions in N-termini of 14 Argonaute genes using ssODN donors and rol-6 coinjection marker
Locus# F1 Rollers positive for insertion by PCRTotal# of F1 Rollers genotyped
wago-11830
wago-21030
ppw-2 (wago-3)1730
wago-42230
wago-5829
sago-2 (wago-6)030
sago-2 (wago-6)a3364
ppw-1 (wago-7)724
sago-1(wago-8)924
hrde-1 (wago-9)330
wago-101024
wago-11848
nrde-3 (wago-12)524
ergo-1424
rde-11424
  • Breakdown of numbers used to derive the %HDR efficiencies plotted in Figure 2C.

  • a Indicates the repeated attempt at targeting sago-2 using the donor described in Figure S1.