Table 2 HDR efficiencies of GFP insertion with blunt-ended PCRs as donors
Locusno. of F1 Rollers (rol-6)
ScreenedGFP+
wago-4541 (1.85%)
sago-2 (wago-6)1191 (0.84%)
ppw-1 (wago-7)761 (1.32%)
  • All the donors consist of 120 bp long homology arms on both ends.