Genotype | Adult overproliferation (%) | L3 EdU+ nucleia |
---|---|---|
gld-1; glp-1 | 0 | Glp |
fog-1 gld-1; glp-1 | ND | Glp |
gld-1 prom-1; glp-1 | 100 | EdU+ |
gld-2 prom-1; glp-1 | 100 | EdU+ |
gld-1 skr-2; glp-1 | 100 | Glp |
gld-2 skr-2; glp-1 | Glpb | Glp |
gld-1 skr-2; glp-1;skr-1(RNAi) | ND | EdU+ |
↵a Young-adult hermaphrodites were assessed for an overproliferation phenotype by EdU incorporation and pH3 staining. Early to mid-L3 hermaphrodites were fed on EdU-labeled bacteria for 30 min and then costained with anti-HIM-3 antibodies as well as anti-PGL-1 antibodies to distinguish germ cells from somatic cells. Glp indicates that all germ cells had entered meiosis. EdU+ indicates germ cells that had undergone EdU incorporation (S phase) during the 30 min pulse, and do not stain for HIM-3.
↵b All germ cells entered meiosis but are arrested with a pachytene-like morphology.