Table 4 The gld-1 or gld-2 SCFPROM-1 double mutant overproliferation defect is due to failure of entry into meiosis
GenotypeAdult overproliferation (%)L3 EdU+ nucleia
gld-1; glp-10Glp
fog-1 gld-1; glp-1NDGlp
gld-1 prom-1; glp-1100EdU+
gld-2 prom-1; glp-1100EdU+
gld-1 skr-2; glp-1100Glp
gld-2 skr-2; glp-1GlpbGlp
gld-1 skr-2; glp-1;skr-1(RNAi)NDEdU+
  • a Young-adult hermaphrodites were assessed for an overproliferation phenotype by EdU incorporation and pH3 staining. Early to mid-L3 hermaphrodites were fed on EdU-labeled bacteria for 30 min and then costained with anti-HIM-3 antibodies as well as anti-PGL-1 antibodies to distinguish germ cells from somatic cells. Glp indicates that all germ cells had entered meiosis. EdU+ indicates germ cells that had undergone EdU incorporation (S phase) during the 30 min pulse, and do not stain for HIM-3.

  • b All germ cells entered meiosis but are arrested with a pachytene-like morphology.