Table 2 Mutation rates using lys2-A14 reversion assay
StrainReversion rate (×10−7) (95% C.I.)Normalized
2μ plasmids
wild-type + pRS424a9.9 (5.8–67)1
wild-type + MSH6-2µa110 (31–647)b11.1
wild-type + 2μ-msh6-KQFF > AAAAa92.5 (67.6–149)b9.3
msh6Δa585 (207–2030)b59.1
msh3Δa37.7 (22.3–49.4)c3.8
wild-type + 2μ-MSH29.2 (7.2–13.8)0.93
wild-type + 2μ-MSH38.3 (4.8–25.0)0.84
wild-type + 2μ-MSH2-MSH6433 (301–537)b44
wild-type + 2μ-MLH1-PMS113.3 (10.0–25.4)1.3
wild-type + 2μ-POL3017.4 (10.4–21.3)1.8
wild-type + pRS4267.3 (4.9–18.4)1
wild-type + 2μ-MSH2-msh6-KQFF > AAAA12.6 (8.2–21.2)1.7
ARS-CEN plasmids
wild-type + pRS316, pRS4149.0 (6.9–17.5)1
wild-type + MSH2, pRS41413.3 (8.2–16.0)1.5
wild-type + MSH6, pRS31615.7 (10.9–18.3)1.7
wild-type + MSH2, MSH616.0 (11.5–21.8)c1.8
  • FY23-derived strains were analyzed for lys2-A14 reversion as described in the Materials and Methods and Table S2. Rates are presented per cell per division. Numbers in parentheses indicate 95% C.I.s. Rates and 95% C.I.s were calculated from 10 to 22 independent cultures.

  • a Published data from Chakraborty et al. (2016).

  • b Significantly different from wild-type using Mann–Whitney U-test (P < 0.01).

  • c Significantly different from wild-type using Mann–Whitney U-test (P < 0.05).