Table 1 Rates of ura3 point mutations and terminal inversions in strains of different genotypes
GenotypeaRate of ura3 point mutations (class 1) (μ × 10−7)Rate of class 1 normalized to wild-typeRate of terminal inversions (class 2) (μ × 10−7)Rate of class 2 normalized to wild-type
Wild-type9.0 (6.1–12)19 (5.9–12)1
elg1Δ11 (5.5–19)1.25.2 (2.1–10)0.6
exo1Δ18 (11–25)1.9*15 (9.1–22)1.2
lig4Δ9.2 (5.4–13)19.2 (5.4–13)1
mms2Δ6.4 (3.4–11)0.719.7 (5.4–13)1.1
mre11Δ1.1 (0.6–3.6)**0.121.3 (0.7–4.2)**0.14
mre11-H125N2.9 (1.4–4.9) **0.327.5 (4.5–9.8)0.83
msh2Δ9 (6.2–13) [0.98]0.980.6 (0.2–1.4)**0.07
msh6Δ8.7 (5.5–14)0.977.8 (4.8–13)0.9
mus81Δ14 (8.2–22)*1.56.9 (3.8–11)0.77
rad1Δ14 (8.2–22)*1.51.4 (0.4–3.0)**0.15
rad18Δ5.6 (2.2–9.1)*0.628.9 (3.5–12)1
rad50Δ3.1 (2.0–4.1)**0.340.9 (0.4–1.8)**0.1
rad51Δ19 (12–26)*2.113 (7.4–20)*1.5
rad52Δ36 (30–58)**41 (0.07–6)*0.1
rad59Δ13 (8.9–19)*1.42.2 (0.9–4.7)**0.25
rev3Δ0.9 (0.4–1.9)**0.14.4 (3.1–7.2)*0.49
rif1Δ11 (8.3–22)1.212 (4.7–16)1.3
rrm3Δ11 (7.6–15)1.22.1 (0.8–4.4)**0.24
sae2Δ3.4 (1.4–6.6)*0.3712 (7.1–18)1.3
sgs1Δ14 (6.3–23)*1.61.3 (0.1–3.8)**0.2
sir2Δ3.2 (1–8) [0.4]*0.427 (20–34)**3
sir3Δ0.55 (0.1–1.9)**0.068.3 (6.3–11)0.92
sml1Δ12 (6.8–19)1.311 (6.3–18)1.3
srs2Δ7.1 (4.6–9.4)0.772.0 (1.0–3.3)**0.22
tel1Δ17 (11–26)*1.923 (11–33)*2.6
tof1Δ3.4 (1.7–6.8)*0.382. (1.7–6.8)*0.38
tof1Δ mre11Δ5.7 (4.1–7.1)*0.30 (0–0.8)**0
top1Δ6.1 (3.8–8.7)0.677.2 (4.6–10)0.8
  • Rates were calculated as described in the text. 95% confidence limits are shown in parentheses and the rates normalized to the wild-type rate are shown in squared brackets. Single asterisks indicate that the rate is outside of the 95% confidence limits of the wild-type strain, and double asterisks show that the rates of the mutant and wild-type strain do not overlap. For rates with double asterisks, underlines and italics indicate whether the rate is higher or lower than the comparable wild-type rates, respectively.

  • a Strain names for the various genotypes listed from top to bottom are: PG329, MD651, MD653, MD687, AM17, AM13, MD740, AM24, MD659, MD658, MD689, AM26, MD691, MD655, MD649, MD686, AM12, MD647, AM14, MD708, MD688, MD657, MD736, MD646, AM15, MD644, MD674, MD734, and MD661. Genotypes shown in bold are the ones used most extensively in developing our models for the genetic regulation of interstitial telomeric sequence-induced instability.