Table 1 Recombination rates in strains overexpressing MMR proteins as measured in the inverted repeat reporter assay
StrainRelevant genotypeRate of His+ recombination (×10−6)Homologous rate/Homeologous ratea
Cβ2-Cβ2pRS426b0.64 (0.47–0.90)c
msh6Δ + pRS4260.62 (0.54–1.7)
msh3Δ + pRS4262.5 (2.1–3.3)d
2µ-MSH6b1.5 (0.68–2.44)e
2µ-msh6-KQFF > AAAAb2.1 (1.38–2.96)d
2µ-MSH3b1.1 (0.66–1.65)d
2µ-MSH2b0.54 (0.26–0.72)
2µ-MLH1-PMS11.57 (0.83–3.11)d
2µ-EXO10.63 (0.42–1.09)
2µ-MSH2-MSH66.0 (4.09–9.40)d
2µ-MSH2-msh6-KQFF > AAAA1.2 (0.90–1.8)d
2µ-POL303.6 (1.0–5.5)d
msh3Δ + 2µ-MSH2-MSH612.7 (10.4–21.8)d
2µ-SGS11.3 (0.61–4.41)e
2µ-MSH6-SGS12.6 (2.1–3.9)d
Cβ2/Cβ2-nspRS426b0.04 (0.02–0.08)16
msh6Δ + pRS4261.0 (0.82–4.5)d0.61
msh3Δ + pRS4261.3 (0.90–2.4)d2.0
2µ-MSH6b0.80 (0.60–1.31)d1.9
2µ-msh6-KQFF > AAAAb0.07 (0.03–0.12)30
2µ-MSH3b0.08 (0.03–0.16)14
2µ-MSH2b0.04 (0.01–0.06)14
2µ-MLH1-PMS10.08 (0.03–0.35)20
2µ-EXO10.04 (0.02–0.09)16
2µ-MSH2-MSH60.11 (0.05–0.23)d56
2µ-MSH2-msh6-KQFF > AAAA0.03 (0.02–0.05)43
2µ-POL300.26 (0.13–0.54)d14
2µ-SGS10.14 (0.10–0.41)d9.6
2µ-MSH6-SGS10.11 (0.02–0.57)24
Cβ2/Cβ2-4LpRS426b0.10 (0.05–0.56)6.4
msh6Δ + pRS4260.10 (0.04–0.16)6.0
msh3Δ + pRS4261.8 (1.3–2.6)d1.4
2µ-MSH6b1.57 (1.12–1.81)d0.96
2µ-msh6-KQFF > AAAAb0.29 (0.11–0.36)7.2
2µ-MSH3b0.06 (0.05–0.09)18
2µ-MSH2b0.09 (0.04–0.13)6.0
2µ-MLH1-PMS10.15 (0.10–0.29)11
2µ-MSH2-MSH60.15 (0.09–0.44)39
msh3Δ + 2µ-MSH2-MSH63.0 (2.3–5.0)d4.2
2µ-MSH2-msh6-KQFF > AAAA0.09 (0.07–0.17)14
2µ-POL300.35 (0.26–0.51)10
2µ-SGS10.12 (0.06–0.21)11
2µ-MSH6-SGS10.32 (0.12–0.81)8.1
  • Homeologous recombination rates and 95% C.I.s were calculated as described in the Materials and Methods. The genotypes of the strains are shown in Table S2. Cβ2/Cβ2, homologous substrate; Cβ2/Cβ2-ns, base–base mismatch substrate; Cβ2/Cβ2-4L, 4-nt loop mismatch substrate.

  • a Homologous rate (Cβ2-Cβ2)/homeologous rate for strains with the same overexpression plasmid.

  • b Published data from Chakraborty et al. (2016).

  • c Numbers in parentheses indicate 95% C.I.s.

  • d Significantly different from wild-type of the same strain (P < 0.01, Mann–Whitney U-test).

  • e Significantly different from wild-type of the same strain (P < 0.05, Mann–Whitney U-test).