Table 1 Sanger sequencing results of imprecise NHEJ junctions
MutationWild-type (%)yku70-R456E (%)pol2-4 (%)yku70-R456E pol2-4 (%)sae2∆ (%)yku70-R456E sae2∆ (%)
CGCAACA(+CA)GT19 (38.8)020 (48.8)3 (7.3)11 (25)0
GCAA(+CAA)CAGT or GCAACA(+ACA)GT16 (32.7)1 (2.1)13 (31.7)014 (31.8)0
GCAACA(+A)GT3 (6.1)00001 (2.2)
GCAAC(+C)AGT02 (4.2)0000
GCAACA(+CACA)GT001 (2.4)01 (2.3)0
GCAA(+AA)CAGT003 (7.3)000
> 5 bp deletion4 (8.2)006 (14.6)11 (25)11 (22)
GCA(−ACA)GT or G(−CAA)CAGT3 (6.1)19 (39.6)022 (53.7)5 (11.4)10 (22.2)
GC(−AACA)GT2 (4.1)00000
GC(−A)ACAGT1 (2)3 (6.3)0005 (11.1)
GC(−AA)CAGT1 (2)001 (2.4)00
GCAA(−CA)GT or GCA(−AC)AGT015 (31.3)04 (9.8)011 (24.4)
GCAA(−CAG)T04 (8.3)02 (4.9)02 (4.4)
(−GC)AACAGT02 (4.2)0000
GCAACA(−G)T0001 (2.4)1 (2.3)0
GCAACACA(−GT)001 (2.4)01 (2.3)1 (2.2)
GCAA(−CAGTA)000002 (4.4)
Unique events, < 5 bp02 (4.2)3 (7.2)2 (4.8)02 (4.4)