Table 1 Yeast strains
StrainGenotypeSource
BY4741MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0Open Biosystems
BY4742MATα his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0Open Biosystems
BY4741 vma2ΔMATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 vma2Δ::KanMxOpen Biosystems
Y3656 vma2ΔMATα can1Δ:: MFA1pr-his3-MFα1pr-Leu2 ura3Δ0 leu2Δ0 his3Δ1 met15Δ0 lys2Δ0 vma2Δ::NatKane laboratory
BY4742 cnb1ΔMATα his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0 cnb1Δ::NatOpen Biosystems
cnb1Δ vma2ΔSpore from cross between BY4741 vma2Δ and BY4742 cnb1ΔThis study
BY4741 reg1ΔMATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 reg1Δ::KanMxOpen Biosystems
BY 4741glc7-12tsMATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 glc7-12ts:: KanMxFrom Charlie Boone
glc7-12ts vma2ΔSpore from cross between Y3656 vma2Δ and BY 4741glc7-12tsThis study
BY4741 rim20ΔMATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 rim20Δ::KanMxOpen Biosystems
BY4741 rim21ΔMATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 rim21Δ::KanMxOpen Biosystems
vma2Δ rim20ΔSpore from cross between Y3656 vma2Δ and BY 4741 rim20ΔThis study
vma2Δ rim21ΔSpore from cross between Y3656 vma2Δ and BY 4741 rim21ΔThis study
BY4741 rim8ΔMATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 rim8Δ::KanMxOpen Biosystems
vma2Δ rim8ΔSpore from cross between Y3656 vma2Δ and BY 4741 rim8ΔKane laboratory
BY4741 ptk2Δ::hygBMATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 ptk2Δ::HygBThis study
BY4741 ptk2Δ::kanMATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 ptk2Δ::kanMxOpen Biosystems
vma2Δ ptk2Δ glc7-12tsSpore from cross between glc7-12ts vma2Δ and BY4741 ptk2Δ::hygBOpen Biosystems
BY4741 crz1ΔMATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 crz1Δ::kanOpen Biosystems
vma2Δ crz1ΔSpore from cross between Y3656 vma2Δ and BY 4741 crz1ΔThis study
SH snf1ΔW303 snf1Δ::URA3From Saul Honigberg
BY 4741 crz1-GFPMATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 crz1-GFPInvitrogen
BY 4741 crz1-GFP vma2ΔMATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 vma2Δ::URA3 crz1-GFPThis study
BY4741 Pma1-007::KanMxMATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 Pma1-007::KanMxOpen Biosystems
BY4741 Pma1-007::HygBMATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 Pma1-007::HygBKane laboratory
BY4741 Rim8 P506A-3HAMATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 Rim8 P506A-3HA-KanMxThis study
vma2Δ Rim8 P506A-3HASpore from cross between Y3656 vma2Δ/ pRS316 vma2-URA3 and BY4741 Rim8 P506A-3HAThis study
vma2Δ Pma1-007Spore from cross between vma2Δ Rim8 P506A-3HA/pRS316 vma2-URA3 and BY4741 Pma1-007::HygBThis study
Pma1-007 Rim8 P506A-3HASpore from cross between vma2Δ Rim8 P506A-3HA/pRS316 vma2-URA3 and BY4741 Pma1-007::HygBThis study
vma2Δ Rim8P506A-3HA Pma1-007Spore from cross between vma2Δ Rim8 P506A-3HA/pRS316 vma2-URA3 and BY4741 Pma1-007::HygBThis study
BY4741 Rim8-3HA::KanMxMATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 Rim8-3HA::KanMxKane laboratory
BY4741 rcy1ΔMATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 rcy1Δ::KanMxOpen Biosystems