Table 1 Summary of stress phenotypes for calcineurin pathway mutants
37°39°5 ng/ml FK506 30°5 ng/ml FK506 37°20 mM DTT0.35 M CaCl25 mg/ml CFW1% Congo red
Wild typeNSSNSSSSNSNSNSNS
cna1SSSSSSWTWTSSSSSSSSSS
cnb1SSSSSSWTWTSSSSSSSSSS
cbp1WTSWTWTWTWTWTWT
cts1SSSSSSSWTSSSS
crz1WTSSWTWTWTSWTWT
pbp1WTWTWTRWTWTWTWT
tif3WTSWTRWTWTSS
puf4SSSSSSWTWTSSWTWT
crz1cbp1WTSWTWTSSSSWTWT
crz1cts1SSSSSSSSSWTSSSSSSSSSSS
crz1puf4SSSSSSWTWTSSSSSSSSSS
cna1pbp1SSSSSWTRSSSSSSSSSS
crz1pbp1WTSWTWTSSSSSS
cna1tif3SSSSSWTRSSSSSSSSSS
crz1tif3WTSWTRSSSSSS
cbp1pbp1SSSSWTWTSSWTWT
  • NS, the strain exhibited no sensitive phenotype under the stress; S, the strain exhibited a mild sensitivity phenotype under the stress; SS, the strain exhibited an intermediate sensitivity phenotype under the stress; SSS, the strain failed to grow under the stress; WT, deletion mutant exhibited a similar stress tolerance phenotype as the wild type; R, deletion mutant exhibited an enhanced stress tolerance phenotype compared to the wild type.