Table 1 Summary of single-step mutants used for construction of double-mutants
SelectionProteinProteinAaNuc.
LabelconditionfunctionnamepositionEmbedded ImageAapositionEmbedded ImageNuc.
AGrowthCapsidF340A → V3587C → T
BSpikeG10N → S4011A → G
CSpikeG171T → A4493A → G
DSpikeG171T → I4494C → T
ESpikeG172V → I4496G → A
FGrowth + heat-shockCapsidF249V → I3310G → A
GCapsidF355P → S3628C → T
HSpikeG38R → C4094C → T
ISpikeG168R → C4484C → T
KGrowth + pH shockCapsidF77I → T2792T → C
LCapsidF393I → V3742A → G
MSpikeG65T → A4175A → G
NSpikeG69N → S4188A → G
OSpikeG171T → A4493A → G
  • Aa, amino acid; Nuc., nucleotide.