Table 3 Percent changes in the amino acid contents of the hybrid PE5;RNAi+ kernels relative to the PE5;RNAi- segregating kernels
Amino Acida% Variation (SD) from PE5;RNAi- Segregating Kernels
PE5;α-PE5;γ-PE5;β-PE5;γ-/α-PE5;γ-/β-
Ala−14.81 (0.001)6.78 (0.002)7.86 (0.006)110.10 (0.005)−2.02 (0.004)
Arg82.72 (0.051)44.06 (0.083)−7.47 (0.030)126.61 (0.038)−4.79 (0.077)
Asp−24.25 (0.016)−32.87 (0.007)−39.86 (0.001)22.92 (0.008)−1.71 (0.051)
Cya16.09 (0.011)−8.39 (0.077)−36.13 (0.020)−3.33 (0.002)−35.62 (0.024)
Glu32.06 (0.019)45.92 (0.051)31.09 (0.012)−59.72 (0.001)12.92 (0.021)
Gly−1.49 (0.019)−30.41 (0.005)−42.01 (0.003)−23.81 (0.004)−16.08 (0.048)
His35.30 (0.013)−29.37 (0.037)−13.81 (0.011)−21.61 (0.006)−29.2 (0.067)
Ile−6.12 (0.005)14.24 (0.020)17.29 (0.015)22.15 (0.001)19.65 (0.024)
Leu−37.57 (0.002)−16.28 (0.055)11.03 (0.065)−33.72 (0.001)29.8 (0.074)
Lys60 (0.043)32.83 (0.129)−20.54 (0.030)128.16 (0.163)−17.26 (0.061)
Phe−40.45 (0.010)−28.47 (0.080)−19.13 (0.058)−51.15 (0.001)44.19 (0.411)
MetS−3.97 (0.018)−4.12 (0.017)−30.64 (0.010)26.38 (0.009)4.64 (0.020)
Pro−7.33 (0.004)−15.20 (0.003)5.25 (0.002)9.39 (0.005)−9.00 (0.002)
Ser−56.69 (0.016)−72.22 (0.003)−76.3 (0.002)−87.03 (0.000)53.33 (0.109)
Thr−47.74 (0.002)−58.28 (0.003)−61.46 (0.002)−61.06 (0.000)−16.24 (0.184)
Tyr−17.65 (0.037)−10.92 (0.018)−14.29 (0.023)91.49 (0.088)−15.13 (0.021)
Val20.7 (0.006)9.79 (0.002)18.35 (0.002)43.88 (0.001)1.05 (0.032)
  • Data shown are means (SD) of three pooled replicates.

  • a Acid hydrolysis of the sample yields the acid stable forms Cya (cysteic acid) and MetS (Met sulfone) from cysteine and Met, respectively.