Table 2 Amino acid composition of transgenic maize kernels from a cross of the maternal PE5 plant with the zein RNAi lines (α-, γ-, β-, γ-/α-, or γ-/β-zein RNAi)
Amino AcidaMol % (SD)
W64Ao2PE5;α-PE5;γ-PE5;β-PE5;γ-/α-PE5;γ/β-
Ala10.06 (0.110)24.38 (1.000)b29.24 (0.855)29.54 (1.915)b23.99 (0.398)b26.83 (1.692)
Arg3.70 (0.133)6.91 (0.745)b5.01 (0.395)3.22 (0.090)5.05 (0.351)b3.31 (0.751)
Asp9.83 (0.613)3.28 (0.439)2.88 (0.332)2.58 (0.098)7.63 (0.110)b4.22 (0.949)
Cya3.21 (0.218)5.36 (0.438)3.57 (0.796)2.49 (0.219)3.58 (0.131)2.51 (0.330)
Glu15.09 (0.266)9.79 (0.870)b10.20 (1.339)b9.16 (0.160)b7.40 (0.625)b7.89 (0.792)
Gly9.28 (0.140)3.30 (0.384)2.38 (0.104)1.98 (0.076)5.22 (0.408)b2.87 (0.704)
His2.93 (0.172)2.59 (0.163)1.35 (0.329)1.64 (0.042)2.24 (0.178)1.35 (0.468)
Ile3.07 (0.032)3.32 (0.240)4.36 (0.523)4.48 (0.454)3.68 (0.067)4.57 (0.578)
Leu7.94 (0.410)5.03 (0.331)b8.30 (1.855)11.00 (1.695)7.09 (0.269)b12.86 (1.591)b
Lys4.21 (0.519)7.25 (0.711)b4.72 (0.799)2.82 (0.165)5.29 (0.389)b2.94 (0.624)
MetS2.57 (0.087)3.39 (0.371)3.72 (0.371)2.69 (0.301)4.95 (0.275)b4.06 (0.389)
Phe2.54 (0.111)0.71 (0.118)1.05 (0.361)1.18 (0.272)1.42 (0.060)b2.11 (0.846)
Pro9.48 (0.514)14.36 (0.944)13.95 (0.767)b17.31 (0.551)12.31 (0.374)b14.97 (0.442)
Ser5.38 (0.085)0.21 (0.049)0.13 (0.007)0.11 (0.006)0.69 (0.070)b0.69 (0.070)
Thr3.96 (0.050)0.50 (0.017)0.44 (0.038)0.40 (0.015)1.32 (0.030)b0.88 (0.439)
Tyr0.23 (0.025)0.42 (0.085)0.35 (0.015)0.34 (0.035)0.30 (0.036)0.34 (0.032)
Val6.46 (0.000)9.27 (0.555)b8.38 (0.150)9.03 (0.139)7.79 (0.119)b7.71 (1.343)
  • Ground corn meal of bulked samples were from kernels segregating for the zein RNAi. Data shown are means (SD) of three pooled replicates. The high-Lys opaque-2 mutant in W64A background, W64Ao2, is added for comparison.

  • a Acid hydrolysis of the sample yields the acid stable forms Cya (cysteic acid) and MetS (Met sulfone) from cysteine and Met, respectively.

  • b Values that are statistically different from their corresponding controls (P < 0.01) of kernels that do not segregate for the RNAi transgene(s).