Table 1 pals-22 effects on transgene reporter expression
Array TypeConstructExpression PatternRelative Intensities of Reporters
Wild-Typepals-22a
Simple arrayric-19prom6::gfpbPan-neuronal++++
myo-3p::gfpBody-wall muscle+++++
cat-2prom::gfpDopaminergic neurons++++++
rab-3prom1::rfpPan-neuronal++++
unc-3p::mCherryCholinergic neurons++++++
tdc-1p::yfpRIC and RIM neurons+++
ric-4p26::yfpcNeuronal+++++
Complex arrayunc-11p8::gfpPan-neuronal++++
cho-1fosmid::yfpCholinergic neurons++++
ric-4fosmid::yfpPan-neuronal++++
Single copylin-4p::yfpUbiquitous++++
myo-3p::mRubyPharyngeal muscle++++
None (endogenous tag)che-1::gfpASE neurons++
  • Transgene expression in wild-type and pals-22 mutants. See Table S1 in File S1 for details on transgenic arrays. Number of plus signs (+) indicates the relative intensity of GFP fluorescence. At least 50 animals examined for each genotype. Unless otherwise indicated, all simple and complex arrays correspond to stable genome integrated transgenes. Single-copy reporters were generated by miniMos. GFP tagging of the che-1 locus was achieved using clustered regularly interspersed short palindromic repeats/Cas9 technology.

  • a pals-22(ot811) mutant background.

  • b otIs381 and otIs380 strains (independently integrated lines).

  • c Extrachromosomal array.