Table 1 Efficiency of FLP-mediated recombination
PromoterCell TypeaStagebObservedcExpectedEfficiency (%)
dat-1Dopaminergic neuronsL2-adult8.0 ± 0.0 (15)8100
dpy-7Hypodermal (vulval cells)L26.0 ± 0.0 (3)6d100
L312.0 ± 0.0 (5)12d100
L422.0 ± 0.0 (2)22d100
elt-2IntestineL120.2 ± 1.0 (14)20 (19–22)101
L231.3 ± 1.1 (9)32 (30–34)98
hlh-8M lineageAdult19.2 ± 4.9 (18)3260
hsp-16.41UbiquitousL4–adultOverlap with mCh::HIS-positive nuclei (20)92e
lag-2Multiple (distal tip cell)L3–adult1.0 ± 0.0 (8)1f100
mec-7Mechanosensory neuronsL4–adult> 12 (8)12NAg
myo-2Pharyngeal muscleAdult33.5 ± 1.6 (14)3791
myo-3Body wall muscleL1–L280.4 ± 1.3 (9)8199
nhr-82Seam cell lineageAdult86.2 ± 22.2 (25)13066
rgef-1Neurons (dopaminergic and GABAergic)L2–L4Overlap with unc-47 and dat-1 markers (15)99h
tph-1Serotonin-producing neuronsAdult6.0 ± 0.0 (14)6100
unc-47GABAergic motor neuronsL2-adult19.2 ± 4.3 (27)2674
  • Quantification of FLP D5 activity in different tissues using the dual color reporter (see Figure 1). For all strains, we observed multiple recombination events in all animals. mCh, mCherry; NA, not applicable; GABA, γ-aminobutyric acid.

  • a Cell types where the promoter is active; cell types used for quantification of recombination efficiency are indicated in brackets.

  • b Developmental stage that was used for determination of recombination efficiency; recombination was also observed in other stages.

  • c Average number of GFP-positive nuclei ± SD; number in brackets: n.

  • d Efficiencies were calculated based on recombination in central Pn.p cells and their descendants: recombination was also observed throughout the hypodermis.

  • e Experiments performed with NLS-FLAG-FLP D5; recombination efficiency was calculated as percentage of nuclei coexpressing mCh::HIS-58 and GFP::HIS-58 after two rounds of heat induction (see Figure 4).

  • f Efficiency was calculated based on recombination in the distal tip cell most proximal to the microscope objective; recombination was also observed in other cell types.

  • g Not applicable; we did not attempt to identify and evaluate the 12 expected nuclei because expression levels were low and because we observed GFP in unexpected neurons.

  • h Efficiency was calculated based on recombination in the dopaminergic neurons in the head and GABAergic motor neurons in the ventral nerve cord; recombination was also observed in other types of neurons.