Table 2 Primers used for site-directed mutagenesis
Primers (5′➔3′)Transversion/DeletionNucleotide position
rad27-M66IACAACGTCACACTTGATCGGTATGTTTTATAGGACG➔C198
GTCCTATAAAACATACCGATCAAGTGTGACGTTGT
rad27-E158DGCGCCAACGGAAGCTGACGCTCAATGTGCTGAGTTGGG➔C474
CCAACTCAGCACATTGAGCGTCAGCTTCCGTTGGCGC
rad27-E176ATATGCCGCAGCAAGTGCAGATATGGACACACTCA➔C512
GAGTGTGTCCATATCTGCACTTGCTGCGGCATA
rad27-NΔ20GATATCCATAT*T*T*T*TCTTCCGGTGTCDeletion of 20 AA after start codon4–64
AATATGGATATCA*A*G*A*GCTTTTTTGGC
  • * , Phosphorothioate bonds.