Table 2 Chemical tagging substrates used in this study
Substrate (abbreviation)FluorophoreExEmBinds toCell permeableSupplierCat. #
SNAP-Cell 647-SiR (SNAP-SiR)SiR645661SNAPm/fYesNew England BiolabsS9102S
SNAP-Surface 549 (SNAP-549)Dyomics DY-549P1560575SNAPm/fNoNew England BiolabsS9112S
CLIP-Surface 488 (CLIP-488)ATTO-TEC 488506526CLIPm/fNoNew England BiolabsS9232S
CLIP-Surface 547 (CLIP-547)Dyomics DY-547554568CLIPm/fNoNew England BiolabsS9233S
HaloTag TMR Ligand (Halo-TMR)TMR555585Halo2/7YesPromegaG8252
HaloTag SiR Ligand (Halo-SiR)SiR645661Halo2/7YesK. Johnssonn/a
  • Commercially available, fluorophore-coupled substrates for SNAP-, CLIP-, and Halo- are listed.