Table 3 Daughter cell T1 and T2 times with and without CLN3
GenotypeT1 (min)T2 (min)
Wild type18 ± 11 (50)18 ± 7 (50)
CLB3db0 ± 1 (50)18 ± 11 (50)
cln326 ± 10 (50)16 ± 7 (50)
cln3 CLB3db0 ± 1 (50)18 ± 9 (50)
  • Times are mean ± SD. Number of cells scored in parentheses. We consider T1 as time from cytokinesis (estimated as Whi5 nuclear entry + 6 min) to Whi5 exit; T2: time from Whi5 exit to bud emergence (Di Talia et al. 2007).