Genotype | No. of germ cells in dauer^{a} | No. of proximal somatic gonadal cells in dauer^{a} |
---|---|---|

(A) daf-2(e1370)^{b} | 34.5 ± 3.5^{*} | 6 ± 0 |

(B) din-1S(rr94); daf-2(e1370)^{b} | 49.7 ± 10.0^{*} | 9.5 ± 4.6^{*} |

(C) daf-2(e1370); daf-12(rh61)^{b} | 40.4 ± 3.7^{*} | 6 ± 0 |

(D) din-1S(rr94); daf-2(e1370); daf-12(rh61)^{b} | 45.0 ± 9.9^{*} | 6 ± 0 |

(E) rrf-1(pk1417); daf-7(e1372) | 31.8 ± 4.4 | 6 ± 0 |

(F) rrf-1(pk1417); daf-7(e1372);E.V. (RNAi)^{c} | 30.6 ± 2.9** | 6 ± 0 |

(G) rrf-1(pk1417); daf-7(e1372); daf-12(RNAi) | 40.1 ± 6.7** | 8.0 ± 6.9 |

(H) rrf-1(pk1417); daf-2(e1370)^{d} | 32.5 ± 3.8 | 6 ± 0 |

(I) rrf-1(pk1417); daf-2(e1370); E.V. (RNAi)^{c}^{,}^{d} | 31.4 ± 2.8*** | 6 ± 0 |

(J) rrf-1(pk1417); daf-2(e1370); daf-12(RNAi)^{d} | 37.9 ± 4.0*** | 6.4 ± 1.8 |

↵*

*P*< 0.0005 (statistical significance) between rows B, C, and D and the control value shown in row A according to a one-tailed*t*-test with unequal variance. ***P*< 0.0005 (statistical significance) between row G and the control value shown in row F according to a one-tailed*t*-test with unequal variance. ****P*< 0.0005 (statistical significance) between row J and the control value shown in row I according to a one-tailed*t*-test with unequal variance.↵a Mean ± SD;

*n*≥ 20.↵b Animals were synchronized as L1 larvae and shifted to the restrictive temperature (25°) for 48 hr before the cell counts to avoid confounding problems with

*daf-12*dauer formation. At this point, although all*daf-2*animals had formed dauer larvae, the*daf-12*were in a transient dauer stage and*din-1S*animals were still in the L2d stage.↵c E.V., empty vector was used as an RNAi control.

↵d Full genotype includes

*qIs56*.