Table 2 din-1S(rr94) is required for normal dauer survival and the timely establishment of cell cycle quiescence in the dauer gonad
GenotypeNo. of germ cells in daueraNo. of proximal somatic gonadal cells in daueraDauer survival in daysa (n)
daf-2(e1370)b33.6 ± 3.96 ± 033.5 ± 8.3 (207)
rr94; daf-2(e1370)b82.0 ± 16.325.4 ± 19.214.4 ± 8.0 (191)
din-1S(dh127); daf-2(e1370)64.2 ± 14.614.9 ± 16.3ND
din-1S(dh149); daf-2(e1370)71.9 ± 15.612.9 ± 15.9ND
din-1S(rr94/dh127); daf-2(e1370)c77.8 ± 15.222.1 ± 15.9ND
din-1S(rr94/dh149); daf-2(e1370)c83.7 ± 12.019.6 ± 19.5ND
daf-2(e1370); E.V. (RNAi)d32.9 ± 2.06 ± 0ND
din-1S(RNAi); daf-2(e1370)72.3 ± 21.820.9 ± 22.0ND
daf-7(e1372)b31.0 ± 3.26 ± 026.3 ± 8.7 (61)
rrf-3(pk1426); daf-7(e1372)34.5 ± 7.06 ± 0ND
rrf-1(pk1417); daf-7(e1372)31.8 ± 4.46 ± 0ND
rr94; daf-7(e1372)b103.3 ± 9.087.6 ± 10.127.2 ± 7.2 (105)
daf-7 (e1372); E.V. (RNAi)d31.2 ± 2.46 ± 0ND
din-1S(RNAi); daf-7(e1372)60.7 ± 13.827.0 ± 11.7*ND
rrf-3(pk1426); daf-7(e1372); E.V. (RNAi)d32.4 ± 2.76 ± 0ND
rrf-3(pk1426); din-1S(RNAi); daf-7(e1372)63.2 ± 9.940.6 ± 19.8*ND
rrf-1(pk1417); daf-7(e1372); E.V. (RNAi)d33.7 ± 2.86 ± 0ND
rrf-1(pk1417); din-1S(RNAi); daf-7(e1372)65.9 ± 24.414.0 ± 12.9*ND
  • * P < 0.05 (statistical significance) between all three strains according to a two-tailed Mann–Whitney U-test.

  • a Mean ± SD; n, sample size, n ≥ 20.

  • b Full genotype includes qIs56.

  • c Full genotype includes qIs56/+. The presence of the transgene was used as a marker for successful mating and for identification of true heterozygous progeny.

  • d E.V., empty vector was used as an RNAi control.