Table 2 Dis3 RNA exonuclease activity is required for normal mitosis
GenotypeAneuploidy (n)Overcondensed chromosomes (n)Mitotic index (n)
Wild-type0% (820)0% (450)14.1% (265/1878)
dis3 + dis3wta0.1% (1/927)0.5% (2/414)20.9% (280/1337)
dis3 + dis3S786Eb5.0% (8/160)44.0% (73/166)26.8% (398/1484)
dis3 + dis3S786A0.2% (2/819)1.3% (6/463)18.8% (314/1671)
P < 1 × 10−5cP < 0.1 × 10−10cdP < 0.0005
  • a dis31/2 mutant larvae that express the wild-type dis3 transgene.

  • b dis31/2 mutant larvae that express the phosphomimetic dis3 transgene.

  • c Fisher’s exact test: comparing dis3 + dis3wt to dis3 + dis3S786E.

  • d Chi-square test: comparing dis3 + dis3wt to dis3 + dis3S786E.