Table 2 Total number of observations in the different simulated trials
RILs per environment (number of standards per environment)a
Number of environments80 (1)400 (4)1000 (10)2000 (20)10,000 (100)25,000 (100)b
108004,00010,00020,000100,000250,000
504,00020,00050,000100,000XX
1008,00040,000100,000XXX
• Boxes marked with X are not simulated.

• a All trials had four replications of the standard genotypes in each environment, and the nonstandard RIL genotypes were tested in four different environments unless otherwise mentioned.

• b This trials had 10 replications of the standard genotypes in each environment, and the nonstandard RIL genotypes were tested in 10 different environments.