Table 3 Percentage of CreA::GFP localized to the nucleus, as determined by microscopy, in the wild-type, creB15, and creC27 background strains in the presence of glucose and Xylan after transfer from glucose to Xylan or after the addition of glucose to Xylan-grown cultures
Strain (%)Glucose 16 hr (%)Xylan 16 hr (%)Glucose 16 hr + Xylan 6 hr (%)Xylan 16 hr + glucose 30 min (%)
CreA::GFP93.630.08.098.3
CreA::GFP creB1586.428.316.5100.0
CreA::GFP creC2729.6**8.1*10.215.0**
  • Between 100 and 200 nuclei were counted for biological duplicates in each condition (*P < 0.01, **P < 0.001 in a one-tailed equal-variance Student’s t-test between the wild-type and mutated strains for each condition).