Table 1 Yeast strains used in this study
StrainGenotypeSource
sst2∆ GEV (JT5919)MATa leu2Δ0 ura3Δ0 his3Δ1 met15Δ0 sst2∆::SpHIS5 leu2Δ0::GEV::NatMXAlvaro et al. (2014)
snf1Δ sst2∆ GEVaMATa leu2Δ0 ura3Δ0 his3Δ1 met15Δ0 leu2Δ0::GEV::NatMX sst2∆::SpHIS5 snf1Δ::KanMX4This study
kin1Δ sst2∆ GEVaMATa leu2Δ0 ura3Δ0 his3Δ1 met15Δ0 leu2Δ0::GEV::NatMX sst2∆::SpHIS5 kin1Δ::KanMX4This study
kin2Δ sst2∆ GEVaMATa leu2Δ0 ura3Δ0 his3Δ1 met15Δ0 leu2Δ0::GEV::NatMX sst2∆::SpHIS5 kin2Δ::KanMX4This study
kin4Δ sst2∆ GEVaMATa leu2Δ0 ura3Δ0 his3Δ1 met15Δ0 leu2Δ0::GEV::NatMX sst2∆::SpHIS5 kin4Δ::KanMX4This study
kcc4Δ sst2∆ GEVaMATa leu2Δ0 ura3Δ0 his3Δ1 met15Δ0 leu2Δ0::GEV::NatMX sst2∆::SpHIS5 kcc4Δ::KanMX4This study
hsl1Δ sst2∆ GEVaMATa leu2Δ0 ura3Δ0 his3Δ1 met15Δ0 leu2Δ0::GEV::NatMX sst2∆::SpHIS5 hsl1Δ::KanMX4This study
frk1Δ sst2∆ GEVaMATa leu2Δ0 ura3Δ0 his3Δ1 met15Δ0 leu2Δ0::GEV::NatMX sst2∆::SpHIS5 frk1Δ::KanMX4This study
gin4Δ sst2∆ GEVaMATa leu2Δ0 ura3Δ0 his3Δ1 met15Δ0 leu2Δ0::GEV::NatMX sst2∆::SpHIS5 gin4Δ::KanMX4This study
cnb1Δ sst2∆ GEV (JT6694)MATa leu2Δ0 ura3Δ0 his3Δ1 met15Δ0 leu2Δ0::GEV::NatMX sst2∆::SpHIS5 cnb1Δ::KanMX4Alvaro et al. (2014)
cna1Δ cna2Δ sst2∆ GEV (JT6695)MATa leu2Δ0 ura3Δ0 his3Δ1 met15Δ0 leu2Δ0::GEV::NatMX sst2∆::SpHIS5 cna1Δ::KanMX4 cna1Δ::KanMX4Alvaro et al. (2014)
BJ5459 GEV (JT6743)MATa ura3-52 trp1 lys2-801 leu2Δ1 his3∆200 pep4∆::HIS3 prb1∆1.6R can1 GAL leu2Δ1::GEV::NatMXAlvaro et al. (2014)
rod1Δ rog3∆ ldb19∆ sst2∆ GEV (JT6716)MATa leu2Δ0 ura3Δ0 his3Δ1 met15Δ0 rod1∆::KanMX4 rog3∆::KanMX4 ldb19∆::NatMX sst2∆::SpHIS5Alvaro et al. (2014)
STE2 sst2∆ GEVaMATa leu2Δ0 ura3Δ0 his3Δ1 met15Δ0 leu2Δ0::GEV::NatMX sst2∆::SpHIS5 STE2::HPHThis study
STE27KR sst2∆ GEVaMATa leu2Δ0 ura3Δ0 his3Δ1 met15Δ0 leu2Δ0::GEV::NatMX sst2∆::SpHIS5 STE27KR::HPHThis study
STE2∆296 sst2∆ GEVaMATa leu2Δ0 ura3Δ0 his3Δ1 met15Δ0 leu2Δ0::GEV::NatMX sst2∆::SpHIS5 STE2∆296::HPHThis study
STE2-mCherry sst2∆ GEVaMATa leu2Δ0 ura3Δ0 his3Δ1 met15Δ0 STE2-mCherry::URA3 leu2Δ0::GEV::NatMX sst2∆::SpHIS5This study
STE27KR-mCherry sst2∆ GEVaMATa leu2Δ0 ura3Δ0 his3Δ1 met15Δ0 STE27KR-mCherry::URA3 leu2Δ0::GEV::NatMX sst2∆::SpHIS5This study
STE27KR-mCherry sst2∆ GEVbMATa leu2Δ0 ura3Δ0 his3Δ1 met15Δ0 STE27KR-mCherry::ura3 leu2Δ0::GEV::NatMX sst2∆::SpHIS5This study
STE2-mCherry bar1∆ (JT6677)MATa leu2Δ0 ura3Δ0 his3Δ1 met15Δ0 STE2-mCherry::URA3 bar1∆::CgLEU2Alvaro et al. (2014)
STE27KR-mCherry bar1∆MATa leu2Δ0 ura3Δ0 his3Δ1 met15Δ0 STE27KR-mCherry::URA3 bar1∆::CgLEU2This study
STE2-mCherry rod1Δ rog3∆ bar1∆ (JT6679)MATa leu2Δ0 ura3Δ0 his3Δ1 met15Δ0 STE2-mCherry::URA3 rod1∆::KanMX4 rog3∆::KanMX4 bar1∆::CgLEU2Alvaro et al. (2014)
STE27KR-mCherry rod1Δ rog3∆ bar1∆MATa leu2Δ0 ura3Δ0 his3Δ1 met15Δ0 STE27KR-mCherry::URA3 rod1∆::KanMX4 rog3∆::KanMX4 bar1∆::CgLEU2This study
bni1Δ sst2∆ GEVaMATa leu2Δ0 ura3Δ0 his3Δ1 met15Δ0 leu2Δ0::GEV::NatMX sst2∆::SpHIS5 bni1Δ::KanMX4This study
  • a To generate a Gal4(1-93)-estrogen receptor (ER)-VP16 chimera (designated GEV)-expressing version of the indicated yeast strain, pACT1-GEV (Veatch et al. 2009; McIsaac et al. 2011) was digested with EcoRV and introduced into the cells of interest by DNA-mediated transformation (Amberg et al. 2005), and nourseothricin-resistant colonies were selected, in which GEV (expressed under control of an ACT1 promoter) is integrated at the leu2Δ0 locus.

  • b The immediately preceding strain was streaked onto plates containing 5-FOA, and a resulting Ura- (ura3) derivative was selected.