Table 3 Genetic interactions of tof1S379A, S626A and xrs2S349A, T675A
Genetic interactions testedaFunctionsGenetic interactions observed
tof1S379A, S626A
 ctf8ΔRequired for sister-chromatid cohesionNo
 srs2ΔDNA helicase; involved in HR and sister-chromatid cohesionNo
 rad9ΔDNA damage-dependent checkpointYes
 dia2ΔF-box protein; required for deactivation of Rad53 checkpoint kinaseYes (suppression)
xrs2S349A, T675A
 sgs1ΔRecQ family DNA helicase; involved in HRNo
 exo1Δ5′-3′ exonuclease; involved in HRYes
 rtt109ΔHistone acetyltransferase; involved in NHEJNo
 yku80ΔKu complex; involved in NHEJ and telomere functionYesb
 clb2ΔB-type cyclin involved in cell cycle progressionNo
 pol32ΔThird subunit of DNA polymerase deltaNo
 rad27ΔFlap endonuclease; required for DNA replication and BERNo
 apn1ΔApurinic/apyrimidinic endonuclease; required for BERNo
 ctf4ΔRequired for sister-chromatid cohesion; DNA replicationNo
 srs2ΔDNA helicase; involved in HR and sister-chromatid cohesionNo
  • a The genetic interactions with tof1S379A, S626A and xrs2S349A, T675A were selected from the manually curated genetic interactions with tof1Δ and xrs2Δ in the Saccharomyces Genome Database (http://www.yeastgenome.org/).

  • b xrs2S349A, T675A yku80Δ showed enhanced growth defects (but not MMS sensitivity) as compared to yku80Δ.