Table 2 Plasmids used in this study
PlasmidGenotypeSource
pEGKGGAL1prom-GSTYeast Deletion Collection (Open Biosystems, Inc.)
, URA3
pEGKG-Rod1GAL1prom-GSTZhu et al. (2000)
, URA3
pEGKG-Rod1315AaGAL1prom-GSTThis study
, URA3
pEGKG-Rod1447AaGAL1prom-GSTThis study
, URA3
pEGKG-Rod1641AaGAL1prom-GSTThis study
, URA3
pEGKG-Rod1706AaGAL1prom-GSTThis study
, URA3
pEGKG-Rod1720AaGAL1prom-GSTThis study
, URA3
pEGKG-Rod1781AaGAL1prom-GSTThis study
, URA3
pEGKG-Rod1447A 641AaGAL1prom-GSTThis study
, URA3
pEGKG-Rod1447A 706AaGAL1prom-GSTThis study
, URA3
pEGKG-Rod13A (Rod1447A 641A 706A)aGAL1prom-GSTThis study
, URA3
pEGKG-Rod14A (Rod1315A 447A 641A 706A)aGAL1prom-GSTThis study
, URA3
pEGKG-Rod15A (Rod1315A 447A 641A 706A 720A)aGAL1prom-GSTThis study
, URA3
pEGKG-Rod16SA (Rod1S315A S447A S641A S706A S720A S781A)aGAL1prom-GSTThis study
, URA3
pGEX6P1-Rod16SE (Rod1S315E S447E S641E S706E S720E S781E)aGAL1prom-GSTThis study
, URA3
pGEX6P1-Rod1ARR (Rod11-402)aGAL1prom-GSTThis study
, URA3
pGEX6P1-Rod1TAIL (Rod1403-837)aGAL1prom-GSTThis study
, URA3
pGEX6P1-Rod11SA ARR (Rod11-402 S315A)aGAL1prom-GSTThis study
, URA3
pGEX6P1-Rod15SA TAIL (Rod1403-837 S447A S641A S706A S720A S781A)aGAL1prom-GSTThis study
, URA3
pEGKG-Rod12SA (Rod1138A 807A)aGAL1prom-GSTThis study
, URA3
pEGKG-Rod12SE (Rod1138E 807E)aGAL1prom-GSTThis study
, URA3
pEGKG-Rod18SA (Rod1S315A S447A S641A S706A S720A S781A 138A 807A)aGAL1prom-GSTThis study
, URA3
pEGKG-Rod1PANA (pJT4954)GAL1prom-GSTAlvaro et al. (2014)
, URA3
pEGKG-Rod1PASA (pJT4955)GAL1prom-GSTAlvaro et al. (2014)
, URA3
pEGKG-Rod1PPxY-less (pJT4956)GAL1prom-GSTAlvaro et al. (2014)
, URA3
pEGKG-Rod1V/PPxY-lessaLDB19promThis study
CEN, HIS3
pEGKG-Rod12A, V/PPxY-lessaLDB19promThis study
CEN, HIS3
pEGKG-Rod16A, V/PPxY-lessaLDB19promThis study
CEN, HIS3
pEGKG-Rod18A, V/PPxY-lessaLDB19promThis study
CEN, HIS3
pEGKG-Rog3GAL1prom-GSTZhu et al. (2000)
, URA3
pEGKG-Rog3∆400GAL1prom-GSTAlvaro et al. (2014)
, URA3
  • a Generated by site-directed mutagenesis (Green and Sambrook 2012b) with synthetic oligonucleotides containing the desired codon alterations (using the wild-type sequences in pRS426 vectors as the template). DNA from the corresponding gene was amplified from genomic DNA by PCR (Green and Sambrook 2012a) and then cloned into pEGKG.