Table 1 S. cerevisiae strains
StrainGenotypeSource
BY4741MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0Open Biosystems
yDH227MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 rad18ΔKANRThis study
yDH350MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 rad9ΔKANRThis study
yDH355MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 sgs1ΔKANRThis study
yDH357MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 xrs2ΔKANRThis study
yDH359MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 rad50ΔKANRThis study
yDH452MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 exo1ΔKANRThis study
yDH455MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 apn1ΔKANRThis study
yDH456MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 ctf4ΔKANRThis study
yDH457MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 ctf8ΔKANRThis study
yDH460MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 mph1ΔKANRThis study
yDH465MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 tof1ΔKANRThis study
yDH492MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 xrs2T675A yku80ΔKANRThis study
yDH513MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 dia2ΔKANRThis study
yDH567MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 xrs2S349A est3ΔKANRThis studya
yDH568MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 xrs2T675A est3ΔKANRThis studya
yDH569MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 xrs2S349A, T675A est3ΔKANRThis studya
yDH576MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 srs2ΔKANRThis study
yDH578MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 ctf4T401A, T411AThis study
yDH587MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 rad50T568AThis study
yDH599MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 apn1S350A, S356AThis study
yDH600MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 est3ΔKANRThis studya
yDH603MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 tof1S379A, S626A ctf8ΔKANRThis study
yDH604MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 tof1S379A, S626A rad9ΔKANRThis study
yDH606MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 xrs2S349A, T675A rad27ΔKANRThis study
yDH607MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 xrs2S349A, T675A pol32ΔKANRThis study
yDH608MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 xrs2S349A, T675A yku80ΔKANRThis study
yDH610MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 pol32ΔKANRThis study
yDH625MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 xrs2S349A, T675A srs2ΔNATRThis study
yDH627MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 rad27ΔKANRThis study
yDH628MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 clb2ΔKANRThis study
yDH629MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 yku80ΔKANRThis study
yDH638MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 xrs2S349A, T675AThis study
yDH641MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 xrs2S349A, T675A sgs1ΔKANRThis study
yDH650MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 rad18T155A, T282, S284AThis study
yDH654MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 xrs2S349A, T675A ctf4ΔKANRThis study
yDH664MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 mph1T540A, S542A, S543AThis study
yDH672MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 tof1S379A, S626AThis study
yDH685MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 xrs2S349A,T675A apn1ΔKANRThis study
yDH687MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 rtt109ΔKANRThis study
yDH690MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 xrs2S349A, T675A rtt109ΔKANRThis study
yDH693MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 tof1S379A, S626A srs2ΔKANRThis study
yDH698MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 xrs2S349A, T675A clb2ΔKANRThis study
yDH700MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 tof1S379A, S626A dia2ΔKANRThis study
yDH702MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 xrs2S349A, T675A rad17ΔKANRThis study
yDH706MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 xrs2S349A, T675A rad24ΔKANRThis study
yDH714MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 xrs2S349A, T675A exo1ΔKANRThis study
yDH730MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 sae2ΔKANRThis study
yDH741MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 xrs2S349A, T675A mec1ΔLEU2 sml1ΔHIS3 sae2ΔKANRThis study
yDH751MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 sae2ΔHIS3This study
yDH752MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 xrs2S349A sae2ΔHIS3This study
yDH753MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 xrs2T675A sae2ΔHIS3This study
yDH754MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 xrs2S349A T675A sae2ΔHIS3This study
yDH755MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 xrs2ΔURA3 sae2ΔHIS3This study
yDH794MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 xrs2S349A yku80ΔKANRThis study
yDH805MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 xrs2S349A T675A tel1ΔKANR sae2ΔHIS3This study
yDH806MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 tof1ΔURA3 sml1ΔHIS3 rad9ΔKANRThis study
yDH807MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 tof1ΔURA3 dia2ΔKANRThis study
  • a EST3 gene was deleted by PCR. The freshly made est3Δ transformants were grown in YPD for 14 hr (approximately eight generations) before being used for experiments and being frozen and stored at −80°C.