| Male genotype | Nonwhite offspring (%) | Expected nonwhite offspring (%) | χ2 (P-value) |
|---|---|---|---|
| Tre1-GAL4/Y; UAS-TraF | 304 (49.1) | 232 (37.5%) | 35.6 (P < 0.0001) |
| Tre1EP496/Y | 65 (9.9) | 329 (50) | 422.1 (P < 0.001) |
| Tre1-GAL4/Y | 141 (43.8) | 161 (50) | 4.7 (P = 0.030) |
w1118 virgin females were presented with equal numbers of mutant/experimental males and w1118 males. While Tre1-GAL4/Y; UAS-TraF males have a competitive advantage over wild-type males, both Tre1EP496/Y and Tre1-GAL4/Y were at a disadvantage when competing with wild-type males. All mating assays were performed in the dark.