Table 4 Heteroduplex rejection efficiency of overexpression strains as determined in survival assays
2μ vectorA-AF-AA-A/F-A
pRS4240.66 ± 0.040.09 ± 0.017.6
MSH60.39 ± 0.04**0.008 ± 0.002**49
msh6-KQFF > AAAA0.37 ± 0.05**0.012 ± 0.003**30
MSH30.55 ± 0.02*0.24 ± 0.02**2.3
MSH20.60 ± 0.070.14 ± 0.02*4.2
MLH1, PMS10.64 ± 0.050.08 ± 0.028.3
EXO10.48 ± 0.02*0.08 ± 0.015.7
POL300.35 ± 0.01**0.05 ± 0.016.6
SGS10.57 ± 0.030.050 ± 0.004*11
TOP3, RMI10.62 ± 0.060.099 ± 0.0046.2
RAD1, RAD100.52 ± 0.03*0.10 ± 0.015.3
SAW10.58 ± 0.040.07 ± 0.017.7
pRS424, pRS4250.50 ± 0.010.10 ± 0.015.0
MSH2, pRS4250.52 ± 0.080.12 ± 0.044.2
MSH6, pRS4240.51 ± 0.030.009 ± 0.005**54
MSH3, pRS4240.52 ± 0.100.20 ± 0.04*2.6
MSH2, MSH60.56 ± 0.060.06 ± 0.019.0
MSH2, MSH30.56 ± 0.050.12 ± 0.034.5
MSH3, MSH60.40 ± 0.050.04 ± 0.01**10
  • Survival of indicated strains expressed as colony forming units on galactose/glucose ± SE of mean for 4 to 18 experiments. Heteroduplex rejection efficiencies are shown as the ratio of A-A to F-A survival. *Significantly different from wild type using t test (P < 0.05). **Significantly different from wild type using t test (P < 0.01).