Table 2 Heteroduplex rejection efficiency of top3ts, rmi1ts, pol30, and msh6 mutants as determined in SSA survival assays
Relevant genotypeTemp.A-AF-AA-A/F-A
Wild type22°0.93 ± 0.090.26 ± 0.053.6
25°0.77 ± 0.130.28 ± 0.062.8
30°0.79 ± 0.110.24 ± 0.063.3
32°0.84 ± 0.070.22 ± 0.053.9
37°0.79 ± 0.100.25 ± 0.073.2
top3ts22°0.70 ± 0.09*0.38 ± 0.01**1.9
25°0.74 ± 0.180.38 ± 0.08*2.0
30°0.75 ± 0.050.48 ± 0.05**1.6
32°0.78 ± 0.040.42 ± 0.04**1.9
37°0.67 ± 0.160.50 ± 0.14**1.4
rmi1ts-222°0.98 ± 0.160.58 ± 0.16*1.7
25°0.95 ± 0.13*0.60 ± 0.18**1.6
37°0.91 ± 0.150.64 ± 0.10**1.4
rmi1ts-322°0.77 ± 0.05*0.41 ± 0.151.9
25°0.83 ± 0.110.41 ± 0.10*2.0
37°0.96 ± 0.08*0.55 ± 0.08**1.8
msh6-KQFF > AAAA30°0.77 ± 0.080.21 ± 0.033.6
pol30-20430°0.70 ± 0.08*0.21 ± 0.033.4
pol30-20130°0.56 ± 0.10**0.17 ± 0.03*3.2
pol30-21630°0.48 ± 0.08**0.13 ± 0.02**3.7
Goldfarb and Alani 2005
Wild type30°0.61 ± 0.120.19 ± 0.033.4
msh6Δ30°0.87 ± 0.020.61 ± 0.061.4
sgs1Δ30°0.79 ± 0.160.75 ± 0.181.1
  • Survival of indicated strains expressed as mean of colony forming units on galactose/glucose ± SD for 3–21 experiments. Heteroduplex rejection efficiency is shown as the ratio of A-A to F-A survival. Significant differences were calculated for data obtained in this study and are indicated as follows: *Significantly different from wild type using t test (P < 0.05). **Significantly different from wild type using t test (P < 0.01). Published data of msh6Δ and sgs1Δ along with wild-type strains from Goldfarb and Alani (2005) are shown for comparison.