Table 3 Mismatch correction of heteroduplexes arising during SSA between F and A flanking sequences
Number of coloniesa
GenotypeMspI+ (F)MspI (A)Mixed (F-A)Percent mixed
Wild-type28825
msh6Δ141878**
pol30-2011731543**
pol30-2041810515
msh6-KQFF > AAAA30837
top3ts-25°106316
top3ts-37°145417
rmi1ts-25°200313
rmi1ts-37°3991220
  • ** Significantly different from wild type using Fisher’s exact test (P < 0.01).

  • a Colonies derived from single unbudded cells grown on media containing galactose were analyzed for the presence of the MspI site (Materials and Methods) present in the ura3-FL100 (F) allele. Mixed colonies contain cells with and without the MspI site.