Table 6 Yeast overexpressing Msh6 display a mutator phenotype in the lys2-A14 reversion assay
StrainReversion rate (×10−7), (95% C.I.)Normalized
Wild type9.9 (5.8–67)1
Wild type +MSH6-2μ110 (31–647)**11.1
Wild type + msh6KQFF > AAAA-2μ92.5 (67.6–149)**9.3
Wild type + msh6F337A-2μ4610 (3270–5650)**464
msh6Δ585 (207–2030)**59.1
msh3Δ37.7 (22.3–49.4)*3.8
  • FY23-derived strains were analyzed for lys2-A14 reversion as described in Materials and Methods and Table 1. Rates are presented per cell division. Numbers in parentheses indicate 95% confidence intervals. Rates and 95% confidence intervals were calculated from 10 independent cultures. *Significantly different from wild type using Mann–Whitney test (P < 0.05). **Significantly different from wild type using Mann–Whitney test (P < 0.01).