Table 1 Comparison of RAD sequencing results from traditional RAD and new RAD protocols applied to the same 96 individual DNA extractions
Normalized sequenced fragmentsNo. of IndividualsAverage sequenced fragments per individualAverage mapped fragments (no clone removal)Average locus coverage (no clone removal)Average mapped fragments (clones removed)Average locus coverage (clones removed)
Traditional RAD122,356,753961,236,438805,659 (65.2%a)8.23253,381 (20.5%a)2.84
New RAD122,356,753961,182,936984,441 (83.2%a)9.77624,642 (52.8%a)7.03
  • a Percentage of average sequenced fragments per individual.