Table 1 Strains used in this work
StrainGenotypeSource or Reference
CML128MATa leu2-3,112 ura3-52 trp1 his4 cande la Torre-Ruiz et al. (2002)
MML344CML128 pkc1::LEU2de la Torre-Ruiz et al. 2002)
YPH499MATa ade2-101 trp-63 leu2-1 ura3-52 his3200 lys2-801P. Hieter gift
YMJ1YPH499 mkk1::KanMX4Jimenez-Sanchez et al. (2007)
YMJ2YPH499 mkk2::SpHIS5Jimenez-Sanchez et al. 2007)
BY4741MATa his3Δ1 leu2Δ met15Δ ura3Brachmann et al. (1998)
BY4742MATα his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0Brachmann et al. (1998)
Y03520BY4741 nbp2::KanMX4EUROSCARF
Y01328BY4741 bck1::KanMX4EUROSCARF
Y02487BY4741 mkk1::KanMX4EUROSCARF
Y02112BY4741 mkk2::KanMX4EUROSCARF
MAR143BY4741 ptc1::nat1Ruiz et al. (2006)
MAR216BY4742 ptc1::nat1Ruiz et al. (2006)
YPL14BY4741 mkk1::KanMX4 ptc1::HIS3This work
YPL15BY4741 mkk2::KanMX4 ptc1::HIS3This work
AGS33BY4741 cch1::KanMX4 ptc1::nat1This work
AGS34BY4741 mid1::KanMX4 ptc1::nat1This work
  • The collection of double ptc1-kinase mutants used for the screen is listed in Table S1.