Table 2 Recombination capability of different chromosomal regions of ustT1 and ustT2
MaleaHermaphroditeaPCR markers (gene allele)Frequencies of recombinants (total adult progeny) Dpy or Rde animals with PCR markers
GR1373 (eri-1(mg366))SHG373 (dpy-13(ust40))mg3660.126 (1635)
GR1373 (eri-1(mg366))SHG374 (ustT1[dpy-13] IV;ustT1[rde-12] V)mg3660.124 (1589)
GR1373 (eri-1(mg366))SHG375 (ustT2[ben-1] III;ustT2[dpy-13]IV)mg3660.125 (2387)
RB1441 (tag-349(ok1644))SHG373 (dpy-13(ust40))ok16440.069 (689)
RB1441 (tag-349(ok1644))SHG374 (ustT1[dpy-13] IV;ustT1[rde-12] V)ok16440 (1680)
RB1441 (tag-349(ok1644))SHG375 (ustT2[ben-1] III;ustT2[dpy-13]IV)ok16440 (2205)
YY166 (ergo-1(gg98))SHG376 (rde-12(ust17))gg980.125 (768)b
YY166 (ergo-1(gg98))SHG374 (ustT1[dpy-13] IV;ustT1[rde-12] V)gg980 (1487)b
RB1870 (clec-49(ok2416))SHG376 (rde-12(ust17))ok24160.102 (740)b
RB1870 (clec-49(ok2416))SHG375 (ustT2[ben-1] III;ustT2[dpy-13]IV)ok24160.110 (556)b
VC2575 (y75b8a.11(ok3346))SHG375 (ustT2[ben-1] III;ustT2[dpy-13]IV)ok33460 (1207)
  • a The genotypes of the strains are showed in parentheses.

  • b The F2 animals suppressing unc-15 RNAi treatment were singled out and genotyped.