Table 1 Human cDNAs that complement essential yeast deletion mutants
Yeast systematic nameYeast standard nameHuman Entrez Gene IDHuman standard nameYeast gene brief descriptiond
YBL020WRFT1a29927SEC61A1Membrane protein required for translocation of Man5GlcNac2-PP-Dol
YBR002CRER279947DHDDSCis-prenyltransferase involved in dolichol synthesis
YBR029CCDS18760CDS2Phosphatidate cytidylyltransferase (CDP-diglyceride synthetase)
YBR109CCMD1b801; 805; 808CALM1; CALM2; CALM3Calmodulin
YBR160WCDC28b983; 1017CDK1; CDK2Cyclin-dependent kinase (CDK) catalytic subunit
YBR252WDUT11854DUTDeoxyuridine triphosphate diphosphatase (dUTPase)
YCR012WPGK1b5230; 5232PGK1c; PGK23-Phosphoglycerate kinase
YDL045CFAD1b80308FLAD1cFlavin adenine dinucleotide (FAD) synthetase
YDL064WUBC9b7329UBE2ISUMO-conjugating enzyme involved in the Smt3p conjugation pathway
YDL120WYFH12395FXNMitochondrial matrix iron chaperone
YDL147WRPN5b5718PSMD12cSubunit of the CSN and 26S proteasome lid complexes
YDL164CCDC9b3978LIG1cDNA ligase found in the nucleus and mitochondria
YDL205CHEM33145HMBSPorphobilinogen deaminase
YDR050CTPI1b7167TPI1Triose phosphate isomerase, abundant glycolytic enzyme
YDR086CSSS123480SEC61GSubunit of the Sec61p translocation complex (Sec61p-Sss1p-Sbh1p)
YDR208WMSS4b8394; 8395PIP5K1A; PIP5K1BcPhosphatidylinositol-4-phosphate 5-kinase
YDR236CFMN155312RFKRiboflavin kinase, produces riboflavin monophosphate (FMN)
YDR404CRPB7b5436POLR2GRNA polymerase II subunit B16
YDR454CGUK12987GUK1Guanylate kinase
YDR510WSMT3b7341SUMO1cUbiquitin-like protein of the SUMO family
YEL026WSNU13b4809NHP2L1RNA binding protein
YEL058WPCM15238PGM3Essential N-acetylglucosamine-phosphate mutase
YER094CPUP3b5691PSMB3Beta 3 subunit of the 20S proteasome
YER112WLSM425804LSM4Lsm (Like Sm) protein
YER133WGLC7b5499; 5501PPP1CA; PPP1CCType 1 serine/threonine protein phosphatase catalytic subunit
YER136WGDI12665GDI2GDP dissociation inhibitor
YFL017CGNA1b64841GNPNAT1cGlucosamine-6-phosphate acetyltransferase
YGL001CERG2650814NSDHLC-3 sterol dehydrogenase
YGL030WRPL30b6156RPL30Ribosomal 60S subunit protein L30
YGL048CRPT65705PSMC5ATPase of the 19S regulatory particle of the 26S proteasome
YGR024CTHG154974THG1LtRNAHis guanylyltransferase
YGR075CPRP3855119PRPF38BUnique component of the U4/U6.U5 tri-snRNP particle
YGR175CERG16713SQLESqualene epoxidase
YGR185CTYS18565YARSCytoplasmic tyrosyl-tRNA synthetase
YGR277CCAB480347COASYSubunit of the CoA-synthesizing protein complex (CoA-SPC)
YGR280CPXR154984PINX1Essential protein involved in rRNA and snoRNA maturation
YIL083CCAB279717PPCSSubunit of the CoA-synthesizing protein complex (CoA-SPC)
YJL097WPHS1201562PTPLBEssential 3-hydroxyacyl-CoA dehydratase of the ER membrane
YJR006WPOL31b5425POLD2Subunit of DNA polymerase delta (polymerase III)
YKL013CARC19b10093ARPC4cSubunit of the ARP2/3 complex
YKL024CURA6b51727CMPK1cUridylate kinase
YKL033WTTI1b9675TTI1Subunit of the ASTRA complex, involved in chromatin remodeling
YKL035WUGP1b7360UGP2cUDP-glucose pyrophosphorylase (UGPase)
YKL145WRPT15701PSMC2ATPase of the 19S regulatory particle of the 26S proteasome
YKL189WHYM151719; 81617CAB39; CAB39LComponent of the RAM signaling network
YML069WPOB3b6749SSRP1Subunit of the heterodimeric FACT complex (Spt16p-Pob3p)
YML077WBET5b58485TRAPPC1Core component of transport protein particle (TRAPP) complexes I-III
YMR208WERG124598MVKMevalonate kinase
YMR308CPSE1b3843IPO5Karyopherin/importin that interacts with the nuclear pore complex
YMR314WPRE5b5682PSMA1Alpha 6 subunit of the 20S proteasome
YOL133WHRT19978RBX1RING-H2 domain core subunit of multiple ubiquitin ligase complexes
YOR143CTHI8027010TPK1Thiamine pyrophosphokinase
YOR149CSMP3b80235PIGZAlpha 1,2-mannosyltransferase
YOR176WHEM152235FECHFerrochelatase
YOR236WDFR11719DHFRDihydrofolate reductase involved in tetrahydrofolate biosynthesis
YPL117CIDI1b3422IDI1cIsopentenyl diphosphate:dimethylallyl diphosphate isomerase
YPR082CDIB1b10907TXNL4Ac17-kDa component of the U4/U6aU5 tri-snRNP
YPR113WPIS110423CDIPTPhosphatidylinositol synthase
  • a Complementation by nonorthologous gene.

  • b Yeast CIN gene.

  • c Complementation identified by both screens.

  • d Brief description obtained from Yeastmine.