Table 2 Classes of Ura+ NAHR clones
NAHR classPhenotypePFGE karyotypeaClones in each Ura+ classb
UraGenChr14/Chr5, 650 kbChr5/Chr14, 700 kbChr5, 570 kbChr14, 780 kbRNH201rnh201Δ
Parent diploidsRNANA22NANA
 1+R111111
 2+S10211417(1)
 3+R1021915(4)
 4+R102251
 5+R101110
 6+R001111(1)
 7+S101110
Total3235(6)
  • NA, not applicable.

  • a The numbers (0, 1, or 2) indicate the number of copies of the indicated chromosomes (parental size Chr5 and Chr14, and the two translocations) determined by measuring the ethidium bromide staining intensity of the corresponding band in PFGE relative to the parental bands in the parent strains (representative examples shown in Figure 3 and Figure S2).

  • b The number of Ura+ clones in each class is shown, and when present, the superscript number in parentheses indicates the number of clones that contained additional chromosomal rearrangements of size other than 650 kb or 700 kb. Six Ura+ clones derived from rnh201Δ diploids contained such uncharacterized rearrangements as observed by PFGE; no rearrangements of unexpected sizes were observed in the wild-type control. Note that all six rearrangements in question had sizes <1200 kb and, therefore, were unlikely to correspond to contractions of the ribosomal DNA tandem repeat cluster on Chr12.