Table 1 S. pombe genomic and cDNA libraries
LibraryInsertMarkerReference
pWH5 (Sau3AI) libraryPartial Sau3AI digest-genomic—10 kbLEU2+Feilotter et al. 1991
pWH5 (HindIII) libraryPartial HindIII digest-genomic—10 kbLEU2+Feilotter et al. 1991
pBG2 libraryPartial Sau3AI digest-genomic—2.9 kbhis3+Ohi et al. 1996
pEA500 libraryPartial Sau3AI digest-genomic—3.9 kbhis7+Wang et al. 2005
pLEV3/SPLE1adh1-driven cDNA (>1-kb transcripts)LEU2+Janoo et al. 2001
pLEV3/SPLE2adh1-driven cDNA (<1.6-kb transcripts)LEU2+Janoo et al. 2001
pREP3X librarynmt1-driven cDNALEU2+Edgar and Norbury, unpublished data
  • Four genomic and three cDNA libraries that have been used to clone S. pombe genes are listed. Information regarding the cloning vector used, the nature of the insert DNA, the selectable marker, and the promoter driving expression of the inserts for the cDNA libraries are given. For genomic libraries, the average size of the inserted DNA is provided. The SPLE1 and SPLE2 cDNA Libraries contain size-selected inserts.