Table 2 Protocol performance with different types of edits
Experiment numberEditTarget locusRecipient strainSize of insertionDistance from cutcrRNA ratio (gof/dpy-10)Template ratio (gof/dpy-10)Percent edits rollers (n)Percent edits nonrollers (n)
AP106RFP insertion with low-efficiency guide RNAglh-1wt750 bp62.5 (29.6/11.8)1.8 (0.83/0.44)47.5% (101)16.6% (120)
AP114GFP insertion with low-efficiency guide RNAhtp-3wt900 bp82.5 (29.6/11.8)1.5 (0.70/0.44)2% (198)0.7% (282)
AP92RFP insertion with GG guide RNAgtbp-1wt700 bp12.5 (39.4/15.7)1.3 (0.56/0.43)20.2% (69)15.8% (126)
AP83GFP insertion at distance from cutgtbp-1wt800 bp282.5 (39.4/15.7)1.1 (0.48/0.43)6.0% (82)3.9% (76)
AP82Ollas insertion at distance from cutgtbp-1wt27 nt282.5 (39.4/15.7)1.1 (0.51/0.43)2.1% (47)0% (47)
AP95Ollas insertion at distance from cut + increase ssODN templategtbp-1wt27 nt282.5 (39.4/15.7)6 (2.58/0.43)8.8% (34)0% (34)
AP103Tagging two genes at once (GFP/RFP)gtbp-1 and fbf-2wt750 and 750 bp1 and 11.8 (14.8/8.1) + 3.6 (29.6/8.1)0.4 (0.20/0.44) + 0.9 (0.41/0.44)28% (57)7.1% (223)
AP115Replacement of gtbp-1 ORF with GFP::H2Bgtbp-1wt1300 bp1 and 01.8 (22.2/11.8) + 1.8 (22.2/11.8)0.9 (0.41/0.44)58.1% (74)7.5% (120)
AP105Replacement of GFP with RFPgtbp-1gtbp-1750 bp0 and 11.8 (22.2/11.8) + 1.8 (22.2/11.8)1.3 (0.59/0.44)26.5% (79)2.7% (144)
::eGFP
::3Xflag
AP105DReplacement of GFP with RFPdeps-1deps-1750 bp0 and 11.8 (22.2/11.8) + 1.8 (22.2/11.8)1.3 (0.59/0.44)21.1% (52)5.9% (135)
::GFP
  • All experiments were conducted as described in Figure 1 by cotargeting dpy-10 and one (or two) target loci as indicated. Concentrations are in picomoles per microliter. gof, gene of interest; Percent edits rollers, % FP+ edits at the target locus among rollers in the top three broods with the most rollers (n= number of rollers screened); Percent edits nonrollers: % FP+ edits at the target locus among nonrollers in the top three broods with the most rollers (n= number of nonrollers screened).