Table 1 Efficiency of mNG^SEC^3xFlag insertion into 8 different endogenous loci
TargetHomology armssgRNADoench et al. sgRNA scoreDistance to cut (bp)No. injectedPrecise insertionsImprecise insertionsFalse positivesEfficiency (%)
ebp-2LongNon-3′GG0.55546060010.0
ebp-2ShortNon-3′GG0.5554483026.3
gex-3LongNon-3′GG0.03919661001.5
gex-3ShortNon-3′GG0.039193530010.0
his-72LongNon-3′GG0.02757290012.5
his-72ShortNon-3′GG0.0275462004.3
his-72Short3′gg0.28140660200.0
mex-5LongNon-3′GG0.20825696008.7
mex-5ShortNon-3′GG0.208252770240.0
mex-5Short3′gg0.42729670210.0
nmy-2LongNon-3′GG0.38267590012.0
nmy-2ShortNon-3′GG0.38261350120.0
oma-2LongNon-3′GG0.1122752002.7
oma-2ShortNon-3′GG0.112236000100.0
oma-2Short3′gg0.39226720130.0
rap-1LongNon-3′GG0.13623783003.8
rap-1ShortNon-3′GG0.136231050130.0
rap-1Short3′gg0.28232610010.0
rga-4LongNon-3′GG0.1504750000.0
rga-4ShortNon-3′GG0.150438603140.0
  • Precise insertions are the desired single-copy integration events confirmed by PCR and/or the correct pattern of mNG fluorescence. Imprecise insertions are insertion events at correct locus, but carry rearrangements within the insertion. False positives are animals that lacked the red fluorescent extrachromosomal array markers but did not carry insertions at the desired locus. Efficiency is defined as the fraction of injected animals yielding precise insertions. See also Figure 4C.