Table 1 Genotype for each sector in 13 analyzed survivors that developed as sectored colonies (white/pink, pink/red, red/white, or white/pink/red)
SurvivoraSector colorGenetic alterationsbBreakpoints of LOH event (SGD coordinates in kb)c
1BPinkTrisomy XII (W)
2APinkTrisomy I (Y); trisomy VI4(W); trisomy X (Y); trisomy XI (Y); terminal LOH XIII (W)cXIII (532–541)
2BRedTrisomy VId (W); monosomy XII (W); interstitial duplication on XIII (Y); terminal duplication on XIV (W)cXIII (B1, 372380; B2, 499505)
cXIV (561568)
3AWhiteTerminal LOH XII (Y)cXII (rDNA, 447–490)
3BPinkTerminal LOH XII (Y)cXII (rDNA, 447–490)
4AWhiteTrisomy+terminal LOH III; monosomy XII (W)cIII (161–165)
4BPinkTrisomy I (Y); UPD VIe; terminal LOH XII (Y)cXII (rDNA, 447–490)
5AWhiteInterstitial deletion IV (W)cIV (B1, 512521; B2, 866889)
5BRedMonosomy III (W); terminal duplication XIIf (W); terminal deletion XIVf (Y); interstitial deletion XV (W)cXII (941947)
cXIV (631634)
cXV (B1, 594601; B2, 703717)
6AWhiteTrisomy I (Y); trisomy II (Y); trisomy+terminal LOH VIII; trisomy+terminal LOH XI; monosomy XII (Y); trisomy XIV (Y)cVIII (254–264); cXI (137–146)
6BPinkTrisomy I (Y); trisomy II (Y); trisomy+terminal LOH VIII; trisomy+terminal LOH XIcVIII (254–264); cXI (134–137)
7AWhiteTerminal LOH XII (Y)cXII (rDNA, 447–490)
7BPinkTerminal LOH XIIg (Y)cXII (368382)
8APinkTrisomy X (W); monosomy XII (W)
8BRedTrisomy X (W); trisomy XII (Y)+ terminal LOHcXII (rDNA, 447–490)
9AWhiteMonosomy III (W); trisomy VI (Y); terminal deletion XII (W)cXII (rDNA, 447–490)
9BPinkMonosomy III (W); trisomy VI (Y); terminal LOH XII (Y)cXII (rDNA, 447–490)
10AWhiteTris III (Y); interstitial duplication III (W)h; trisomy X (Y); interstitial LOH event X (Y)cIII (BP1, 8295; BP2, 167175)
cX (BP1, 143–146; BP2, 167–170)
10BPinkTerminal LOH XII (Y)cXII (rDNA, 418–491)i
10CRedTerminal LOH IV (W); terminal LOH XII (Y)cIV (1334–1337); cXII (rDNA, 447–490)
11AWhiteTerminal LOH XII (W)cXII (rDNA, 447–490)
11CRedTerminal duplication XIIg (W)cXII (215221)j
12BRedTrisomy+terminal LOH V (Y); trisomy VI (W); trisomy X (W)cV (547–551)
13AWhiteTerminal LOH IV (Y)cIV (447–483)
13BRedTerminal LOH IV (W)cIV (450–453)
  • a Each sectored colony is given a different number. A, B, and C indicate different sectors from the same survivor colony.

  • b For trisomy and monosomy events, the letters W (W303-1A) and Y (YJM789) indicate the homolog that is duplicated or lost; similarly, for deletions/duplications, W and Y indicate the homolog from which sequences were duplicated or lost. “Terminal LOH” indicates loss of heterozygosity extending to the telomere. These events have a single breakpoint whereas interstitial LOH events have two breakpoints. For LOH events, letters in parentheses show the homolog that is the source of the homozygous SNPs.

  • c SGD coordinates of breakpoints between heterozygous and homozygous SNPs for various types of LOH events. Boldface indicates that there is a Ty or δ/σ-element located between the indicated coordinates.

  • d For these chromosomes, the hybridization ratio suggests that the DNA sample was derived from a mixture of two subpopulations of cells, one with the alteration and one without the alteration.

  • e UPD indicates uniparental disomy (Andersen and Petes 2012). In this strain, there were two copies of the YJM789-derived homolog and none of the W303-1A-derived homolog.

  • f As discussed in the text, the deletion on chromosome XIV may be related to the duplication on chromosome XII.

  • g The breakpoint of this event on XII is not associated with the rDNA.

  • h This duplication includes the centromere of III.

  • i The breakpoints in this rearrangement were unclear but occurred near the rDNA.

  • j This region has a δ-element in S288c, but in some strains has a Ty (Argueso et al. 2008). The duplication contains CEN12.