Table 1 Outlier selections from primary and secondary screens
Lines obtainedLines screenedPrimary screen hitsSecondary screen hits
36553207964600
  • Outliers from the primary and secondary screen of the KK RNAi library were made based on the distribution of performance index scores of the population shown in Figure 1. The average PI of the first 800 lines, 200 tested by each experimenter, was 0.38 with a standard deviation of 0.14. Lines deviating by ∼1 standard deviation from the mean (0.24< and >0.52) were kept as putative hits.