Table 2 Outliers from the secondary screen with an increased PI
Transformant IDCG no.Drosophila genePrimarySecondaryPhysical abnormalityMean activity differenceAct. sig.
PI and SEM linePI and SEM line
100151CG10483CG104830.64 ± 0.100.62 ± 0.03−11.66
100363CG42614scribble0.85 ± 0.040.63 ± 0.047.40
100624CG13521roundabout0.55 ± 0.060.61 ± 0.06−3.13
100706CG1470Guanylyl cyclase β-subunit at 100B0.62 ± 0.070.61 ± 0.02−4.07
100721CG11326Thrombospondin0.65 ± 0.070.53 ± 0.104.62
100727CG8715lingerer0.65 ± 0.120.54 ± 0.0329.81***
101189CG42244Octβ3R0.67 ± 0.030.6 ± 0.08−0.38
102058CG1128α-Esterase-90.65 ± 0.040.60 ± 0.042.12
102373CG3217CKII-α subunit interactor-30.66 ± 0.120.60 ± 0.021.53
102563CR17025CR170250.56 ± 0.100.70 ± 0.051.65
102816CG12806tipE homolog 10.63 ± 0.080.62 ± 0.0319.09***
103625CG6746CG67460.61 ± 0.070.53 ± 0.02−5.76
103767CG13387embargoed0.74 ± 0.030.64 ± 0.0715.15**
104255CG6800N/A0.65 ± 0.090.63 ± 0.06−3.04
104262CG6658Ugt86Di0.63 ± 0.010.61 ± 0.06−7.79
104763CG10251portabella0.57 ± 0.060.72 ± 0.025.78
104782CG8418Ras which interacts with calmodulin0.68 ± 0.060.65 ± 0.071.40
104796CG5036CG50360.60 ± 0.060.52 ± 0.048.90
105374CG11734HERC20.62 ± 0.070.60 ± 0.082.37
105624CG42783atypical protein kinase C0.67 ± 0.070.59 ± 0.074.53
105996CG14015CG140150.65 ± 0.030.60 ± 0.092.37
106046CG3423Stromalin0.72 ± 0.040.61 ± 0.024.09
106248CG10238Molybdopterin synthase 20.66 ± 0.050.48 ± 0.01−4.19
106255CG7125Protein Kinase D0.65 ± 0.030.6 ± 0.022.09
106555CG7442CG74420.74 ± 0.070.6 ± 0.0513.75*
106641CG8808Pyruvate dehydrogenase kinase0.59 ± 0.090.63 ± 0.075.43
106642CG9375Ras oncogene at 85D0.56 ± 0.070.53 ± 0.058.06
106805CG9044CG90440.58 ± 0.170.56 ± 0.116.12
107037CG43720sickie0.67 ± 0.030.61 ± 0.066.90
107047CG6860Leucine-rich-repeats and calponin homology domain protein0.62 ± 0.040.58 ± 0.067.18
107297CG5941CG59410.63 ± 0.070.64 ± 0.0612.78
107725CG9481UDP-glycosyltransferase 37b10.6 ± 0.060.5 ± 0.03−1.88
108011CG13893CG138930.58 ± 0.110.6 ± 0.06−0.16
108453CG1657CG16570.60 ± 0.020.67 ± 0.0610.90
108694CG6384Centrosomal protein 190kD0.58 ± 0.110.69 ± 0.06−2.63
108836CG1989Yippee0.56 ± 0.090.55 ± 0.065.93
109290CG3654Jumonji, AT rich interactive domain 20.61 ± 0.040.63 ± 0.015.40
110045CG30443Optix-binding protein0.65 ± 0.060.61 ± 0.14−0.91
110197CG5429Autophagy-specific gene 60.63 ± 0.070.54 ± 0.11−2.79
110205CG12178Na+/H+ hydrogen exchanger 10.62 ± 0.130.60 ± 0.08−4.72
110310CG11072Maternal gene required for meiosis0.53 ± 0.020.58 ± 0.028.84
110788CG6438amontillado0.59 ± 0.050.54 ± 0.092.87
  • The performance index (PI) was calculated as the number of flies avoiding the CS+ minus those avoiding the CS over the total in both T-maze arms [(CS) − (CS+)]/[(CS) + (CS+)]. The primary screen was performed with n = 3 or 4, the secondary screen at a later date with n = 4. Physical abnormalities, such as misshapen wings or lethargic behavior, are noted by (+) in companion Table S2. Mean activity difference for the 2-min window simulating the decision period during testing was calculated from the TriKinetics Monitoring System (mean activity score 60100) − (mean activity score experimental line). The mean activity score for the 60100 control line was 44.43 ± 0.42 (n = 552). Differences in activity (act. sig.) were determined using ANOVA followed by Dunnett’s post hoc test. *P < 0.05, **P < 0.01, ***P < 0.001.