Table 1 The genotypes, brief description of the mode of action, and the observed efficiencies of the effector genes used in this study
NameGenotypeInsertion chromosome(s)ReferenceMechanismEfficiency in OA/TA neuronsEfficiency in photoreceptor neurons
Cell ablation
UAS-reaper (rpr)w1118 P(UAS-rpr.C)271Zhou et al. (1997)Binding to IAPs and thus inducing cell death++++
UAS-grimw*; P{UAS-grim.N}22Wing et al. (1998)Binding to IAPs and thus inducing cell death++++
UAS-hid,rprw, P[UAS-hid], P[UAS-rpr]1Zhou et al. (1997)Bindingto IAPs and thus inducing cell death++++++
UAS-DTIP{UAS-Cbβ\DT-A.I}14/TM33Bellen et al. (1992)Inhibits protein synthesis+++++
Neuronal silencing
UAS-shibirets (shits)w; P{UAS-shits1.K}33Kitamoto (2001)Temperature-sensitive dynamin impairs vesicle recycling++++++
UAS-TNTEw; P{UAS-TeTxLC.tnt}E22Sweeney et al. (1995)Cleaves n-Syb and impairs vesicle docking
UAS-Kir2.1w*; P{UAS-Hsap\KCNJ2.EGFP}12Baines et al. (2001)Inward rectifying K+ channel prevents membrane depolarization++++++
UAS-ΔOrky1 w*; P{UAS-Ork1.Δ-C}22Nitabach et al. (2002)Outward rectifying K+ channel prevents membrane depolarization+++
Neuronal activation
UAS-TRPM8yw; UAS-TRPM8 C4-D;UAS-TRPM8 C4-A, UAS-TRPM8 C1-A2)2; 3Peabody et al. (2009)Temperature-dependent cation channel opens in response to ∼12°–16° → cell depolarization
UAS-TRPA1w; P{UAS-TRPA1(B).K}attP162Rosenzweig et al. (2005)Temperature-dependent cation channel opens in response to ∼29° → cell depolarization++++++
UAS-PacαP{UAS-Zzzz\PACα.SL}3Schröder-Lang et al. (2007)Eukaryotic photoactivatable adenylyl cyclase increasing intracellular cAMPND
UAS-bPacw*; P{UAS-bPAC.S}2Stierl et al. (2011)Bacterial photoactivatable adenylyl cyclase increasing intracellular cAMPND
UAS-ChR2(a) P{UAS-ChR2.S}2; P{UAS-ChR2.S}3 (ChR2-wt)(a) 2; 3(a) Nagel et al. (2003)Photoactivatable cation channel (470 nm) → cell depolarization(a) +ND
(b) UAS-ChR2-XXL/cyo:GFP(b) 2(b) Dawydow et al. (2014)(b) +++
UAS-NaChBacy1 w*; P{UAS-NaChBac-EGFP}1/TM3, Sb13Nitabach et al. (2006)Bacterial sodium channel increases sodium conductance → cell depolarization++++
Gal4 lines
Tdc2-Gal4w*; P{Tdc2-GAL4.C}22Cole et al. (2005)Drives expression in octopaminergic/tyraminergic neurons
lGMR-Gal4y1 w*; wgSp-1/CyO; P{longGMR-GAL4}3/TM23Moses and Rubin (1991)Drives expression in photoreceptor cells