Table 1 Mutations observed in the original ∆8 strain compared to isogenic WT cells
No.Fraction
Type of substitution
Transition
 A:T > G:C9560.36
 G:C > A:T9420.36
Transversion
 A:T > T:A2390.09
 A:T > C:G1640.06
 G:C > T:A1710.07
 G:C > C:G1610.06
Total2633
Substitutions
Position
 Noncoding11610.44
 Coding14720.56
Coding sequences
 Nonsense40.0015
 Missense4270.29
 Silent10410.71
Indels
 Noncoding1170.78
 Coding330.22
  • Distribution of 2633 point mutations and 150 indels identified in the initial ∆8 strain. The entire list of mutations, together with positions and nucleotide changes, is shown in File S1.