Table 2 Oocytes prematurely enter M phase in lin-41 mutants
GenotypeTemperatureNo. of proximal M-phase nucleia% Fixed gonads with M-phase nucleia
Immediately after pachytene (%)Proximally (%)
lin-41(n2914)20°14.2 ± 9.7 (n = 13)63 (n = 16)100 (n = 16)
lin-41(tn1505)20°ND90 (n = 21)100 (n = 21)
lin-41(tn1487 ts)b25°c1.5 ± 1.4 (n = 20)25 (n = 20)85 (n = 20)
lin-41(tn1487 ts)b22°c1.2 ± 1.7 (n = 22)0 (n = 22)54 (n = 22)
lin-41(tn1487 ts)b15°0.2 ± 0.4 (n = 14)0 (n = 14)21 (n = 14)
lin-41(tn1487 ts)b22°d0.6 ± 0.6 (n = 24)0 (n = 24)54 (n = 24)
lin-41(tn1487 ts); oma-1(zu405te33); oma-2(te51)b22°d0 ± 0 (n = 27)0 (n = 27)0 (n = 27)
lin-41(tn1487 ts); fog-2(oz40)e22°f0.5 ± 0.8 (n = 28)0 (n = 28)32 (n = 28)
  • a Nuclei that have undergone NEBD and are positive for phospho-histone H3 (Ser10).

  • b lin-41(tn1487 ts) homozygous parent.

  • c Animals were upshifted from 15° as embryos.

  • d Animals were upshifted from 15° as L4 larvae.

  • e lin-41(tn1487 ts) heterozygous parent.

  • f Animals were upshifted from 15° as L2–L4 larvae.